PPAR-γ基因多态性与妊娠期糖尿病相关性的初步探讨  

Preliminary Discussion on the Association of Single Nucleotide Polymorphism in PPARγ Gene with Gestational Diabetes Mellitus

作　　者：刘文惠[1] 杨晓红[2] 张元珍[1] 

机构地区：[1]武汉大学中南医院妇产科,湖北武汉430071 [2]湖北省孝感市中心医院妇产科,湖北孝感432100

出　　处：《武汉大学学报（医学版）》2014年第1期69-73,共5页Medical Journal of Wuhan University

基　　金：湖北省卫生厅资助项目(编号:JX2B36)

摘　　要：目的:探讨PPARγ基因Pro12Ala多态性与妊娠期糖尿病之间的关系。方法:应用聚合酶链式反应-限制性内切酶技术,对104例妊娠期糖尿病(GDM)妇女和120例正常对照者PPARγ2基因Pro12Ala多态性位点进行基因分型。结果:PPARγ2Pro12Ala多态性的基因型频率PP、PA、AA,在GDM组和正常对照组中分别为92.4%、7.6%、0.0%以及85%、13.3%、1.7%。P等位基因和A等位基因在GDM组和对照组的频率分布分别为96.2%、91.7%和3.8%、8.3%。两组间的基因型和等位基因频率均无显著性差异(P>0.05)。在GDM组中,PPARγ2Pro12Ala多态性与体重指数呈正相关(P<0.01),而与空腹血糖、胰岛素、甘油三脂、总胆固醇无相关性。PPARγ2Pro12Ala多态性可能与GDM妇女肥胖的发生有一定的相关性,但与GDM无明显联系。Objective： To explore whether a peroxisome proliferator-activator receptor-γ2 （PPARγ2） Prol2Ala polymorphism was associated with gestational diabetes mellitus （GDM）. Methods： In this case-control study, anthropometric and metabolic variables of 120 non-diabetic pregnant women and of 104 women who were diagnosed as GDM according to 75 g oral glucose tolerance test （OGTT） were compared on the basis of PPAR-y2 polymorphism by PCR-RFLP. Results： The genotype frequencies （PP,PA,AA） of the Prol2Ala variant in GDM and control group were 92.4%,7.6 % ,0.0% and 85 %,13.3%,1.7%. Frequencies of P allele and A allele were 96.2% vs 91.7% and 3.8% vs 8.3% in GDM group vs control group. There were no differences in geno- type and allele frequencie between these two groups（P〉0.05）. But in the GDM group, there has a significant association between geneotype and the body mass index,while there was no differ- ence between geneotype and the FBS, FINS, TG and HOMA-IR. Conclusion： The PPAR-γ2 Prol2Ala polymorphism was not significantly associated with GDM, but it tends to increase the risk of obesity in GDM women.

关 键 词：妊娠期糖尿病 PPAR-Γ 基因多态性 

分 类 号：R594.8[医药卫生—内科学]