Ag85A、Ag85B和HspX蛋白特异性细胞免疫反应对结核性胸膜炎的辅助诊断价值  

Diagnosis of Tuberculosis Pleurisy Based on Mycobacterium Tuberculosis Ag85A,Ag85B and HspX Specific Cellular Immune Response

作　　者：陆兰英 李佺 王卫华 

机构地区：[1]武汉市结核病防治所,湖北武汉430030

出　　处：《武汉大学学报（医学版）》2014年第1期106-109,共4页Medical Journal of Wuhan University

基　　金：武汉市卫生局资助课题(编号:WG10B03)

摘　　要：目的:探讨3种结核分枝杆菌保守抗原Ag85A、Ag85B和HspX抗原特异性细胞免疫反应对结核性胸膜炎的辅助诊断价值。方法:收集我院收治的胸腔积液患者30例,根据胸水性质,分为结核性胸膜炎组20例,非结核性胸膜炎组10例。收集胸水样本,分离胸水样本中细胞成分。原核表达、纯化并鉴定结核分枝杆菌Ag85A、Ag85B、HspX蛋白。用Ag85A、Ag85B及HspX蛋白分别刺激2组患者的胸水细胞成分,通过ELISA技术检测IFN-γ表达水平并进行组间比较。结果:Ag85A蛋白刺激后,非结核性胸膜炎组IFN-γ为(15.50±2.41)ng/L,结核性胸膜炎组IFN-γ为(1 775.00±362.86)ng/L;Ag85B蛋白刺激后,非结核性胸膜炎组IFN-γ为(14.89±2.30)ng/L,结核性胸膜炎组IFN-γ为(2 442.50±418.94)ng/L;HspX蛋白刺激后,非结核性胸膜炎组IFN-γ为(14.20±2.30)ng/L,结核性胸膜炎组IFN-γ为(3 570.00±496.41)ng/L;两组比较均P<0.01。结核性胸膜炎组中,不同患者对不同抗原刺激所产生的IFN-γ水平有一定差别。结论:结核性胸膜炎患者胸水中的细胞成分经Ag85A、Ag85B及HspX蛋白刺激后,IFN-γ水平均不同程度明显升高,该方法对结核性胸膜炎有一定的辅助诊断价值。Objective. To construct a method to diagnosis tuberculosis pleurisy based on specific cellular immune response induced by Mycobacterium tuberculosis Ag85A, Ag85B, and HspX antigens. Methods： Thirty samples of pleurisy, including twenty tuberculosis pleurisy samples （group A） and ten non-tuberculosis pleurisy samples （group B）, were collected to separate cell component and supernatant. Mycobacterium tuberculosis Ag85A, Ag85B, and HspX antigens were ex- pressed in pET30A procaryotic expression system and purified. Cell components of samples were stimulated with Ag85A, Ag85B, and HspX proteins for 72 h and the concentrations of induced IFN-γ were detected with human IFN-γ/ELISA kit. Results： In group A, the level of IFN-γ were （1 775.00±362.86） ng/L, （2 442.50±418.94） ng/L and （3 570.00±496.41） ng/L respectively significant increase of IFN-γ after stimulation method is potent to be developed as a kind of culosis pleurisy. with Ag85A, Ag85B and HspX antigens, and this specific and fast method for the diagnosis of tuberculosis pleurisy.

关 键 词：结核性胸膜炎 抗原 细胞免疫反应 IFN-Γ 

分 类 号：R521.7[医药卫生—内科学] R446.6[医药卫生—临床医学]