Molecular identification of Sporothrix clinical isolates in China  

作　　者：Ting-ting LIU Ke ZHANG Xun ZHOU 

机构地区：[1]Department of Dermatology, Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China [2]State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2014年第1期100-108,共9页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China （No. 31270062） and the Chongqing Science and Technology Commission （No. cstc2011jjA10089）, China

摘　　要：In this study, we investigated the molecular phylogeny of 64 clinical isolates which were identified as Sporothrix schenckii sensu lato by morphological identification. All of the strains were isolates from patients from several provinces in China. The phylogeny was inferred by DNA sequence analyses based on datasets of the ribo- somal internal transcribed spacer （ITS） and a combined ITS and partial 13-tubulin region. Reference sequences were retrieved from GenBank. Results showed that all of the isolates were clustered in a distinct clade with a type of Spo- rothrix globosa. Our analysis showed that S. globosa is the causal agent of the tested sporotrichosis in China, rather than S. schenckii that was generally believed to be the case. The existence of S. schenckii in China remains to be confirmed. This study improved our understanding of the distribution of the species in S. schenckii complex.研究目的:明确中国地区孢子丝菌病的致病菌种。创新要点:构建中国地区临床孢子丝菌系统进化树,用分子生物学方法对致病菌种进行重新鉴定。研究方法:多基因测序和系统发育分析。重要结论:球形孢子丝菌可能为中国地区孢子丝菌病的主要病原菌,申克孢子丝菌在中国地区是否存在尚需进一步验证。

关 键 词：Internal transcribed spacer （ITS） DIAGNOSIS Sporothrix globosa TAXONOMY PHYLOGENY 

分 类 号：R75[医药卫生—皮肤病学与性病学]