健脾清化方对阿霉素肾病大鼠免疫炎症损伤的作用机制  

Effects of Jianpi Qinghua Decoctions on Immune Inflammatory Injury in Adriamycin-induced Nephropathic Rats

作　　者：马晓红[1,2,3] 何立群[1,2,3] 

机构地区：[1]上海中医药大学附属曙光医院肾内科,上海201203 [2]上海市中医临床重点实验室,上海201203 [3]上海中医药大学,上海201210

出　　处：《四川大学学报（医学版）》2014年第1期19-23,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金项目(No.81173219);科技部中医药行业科研专项(No.201007005);上海市科委创新行动计划项目(No.11DZ1973100);上海市中医药事业发展三年行动计划项目(No.ZYSNXD-CC-YJXYY);上海市教育委员会E-研究院建设计划资助项目(No.E03008);上海高校创新团队建设项目资助

摘　　要：目的从细胞免疫介导的炎症损伤角度,探讨健脾清化方对阿霉素致局灶节段硬化(focal segmental glomurular sclerosis,FSGS)模型大鼠FSGS的影响。方法左侧肾切除加尾静脉注射阿霉素致大鼠肾脏FSGS,观察健脾清化方对模型大鼠脾淋巴细胞CD4+/CD8+比值,肾小管间质纤维连接蛋白(FN)mRNA、Ⅲ型胶原(ColⅢ)mRNA、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)6表达的影响。结果健脾清化方能明显降低模型鼠脾淋巴细胞CD4+/CD8+比值,肾小管间质FN mRNA、ColⅢmRNA、TNF-α、IL6水平。结论健脾清化方对阿霉素肾病模型大鼠肾脏FSGS有一定的改善作用,该机制可能与健脾清化方抑制细胞免疫介导的炎症损伤有关。Objective To investigate the effects of Jianpi Qinghua Decoctions on the inflammation injury mediated by the cellular immunity in the focal segmental glomurular Sclerosis （FSGS） nephropathy rats. Methods The FSGS nephropathy rat model was established by the method of intravenous injection of Adriamycin after the removal of one kidney. After the treatment of Jianpi Qinghua Decoctions,the blood, spleen and kidney samples of each rat were collected for the detection of splenocytes CD4＋/CD8＋ ratio,renal tubulointerstitial fibronectin （FN） mRNA, Col HI mRNA, and the expression levels of TNF-α and IL6. Results The treatment of Jianpi Qinghua Decoctions decreased the levels of CD4＋/CD8＋,tubulointerstitial FN mRNA, Col HI mRNA, TNF-α and IL6 significantly in FSGS nephropathy rats. Conclusion Jianpi Qinghua Decoctions could improve renal FSGS damage in adriamycin-induced nephropathy rats.

关 键 词：健脾清化方 CD4^+ CD8^+ 局灶节段性硬化 

分 类 号：R285.5[医药卫生—中药学]