人脐带间充质干细胞治疗大鼠糖尿病神经性足溃疡的疗效及VEGF表达变化  

Human Umbilic Mesenchymal Stronmal Cells Repairs Diabetic Foot in Rats Associated with VEGF Expressional Change

作　　者：王瑛[1] 但齐琴[2] 王庆萍[1] 周宁[1] 金醒眆 侯宗柳[1] 彭葆坤[1] 王廷华[2,3] 

机构地区：[1]昆明医科大学附属延安医院,昆明650051 [2]昆明医科大学神经科学研究所,昆明650031 [3]四川大学华西医院转化神经科学中心,成都610041

出　　处：《四川大学学报（医学版）》2014年第1期29-33,共5页Journal of Sichuan University(Medical Sciences)

基　　金：云南省自然科学基金(No.2009CD203)资助

摘　　要：目的调查人脐带间充质干细胞(HUMSC)治疗对糖尿病大鼠足溃疡中血管内皮生长因子(VEGF)表达变化的影响。方法用高脂饲料喂养的成年SD大鼠4周并给予链脲佐菌素腹腔注射(首次注射25mg/kg,1周后再次注射40mg/kg)制作糖尿病模型,然后挤压坐骨神经,并在足部制作切口涂抹金黄色葡萄球菌诱导大鼠糖尿病神经性足溃疡形成;随后将培养鉴定的HUMSC涂于溃疡表面,治疗10d。测量溃疡体积,RT-PCR和Western blot测定溃疡组织VEGF基因和蛋白表达变化,免疫组化确定VEGF细胞定位及血管密度变化。结果 HUMSC移植促进足溃疡体积明显缩小(P<0.05),VEGF基因和蛋白水平表达明显上调(P<0.05);VEGF主要定位于血管;溃疡周组织血管密度明显增加(P<0.05)。结论 HUMSC移植可能通过调节VEGF的有效表达促进糖尿病神经性足溃疡修复。Objectitve To explore the mechanism of human umbilicmesenchymal cells （ HUMSCs） implantation for the treatment of diabetic foot in rats associate with vascular endothelia growth factor （VEGF） expression changes. Methods After diabetic foot model in rats were established by administration of streptozotozin （STZ） in intraperitoneal injection （2 weeks）,ulceration in foot was induced by incision injury combined with swearing staphylococcus aureas. Then, HUMSCs were smeared on the ulceration of foot in diabetic rats. Ten days later, the densities of blood vessel and the level of VEGF expression were determined by using immunohistochemistry,RT-PCR and Western blot. Results HUMSC grafts reduced significantly the volume of ulceration in diabetic foot rats （P〈0, 05）. RT-PCR and Western blot showed that VEGF and its mRNA were significantly upregulated （P〈0.05）. VEGF immunstaining was found in blood vessels and the densities of blood vessels in HUMSC group were increased significantly （P〈0.05）. Conclusion HUMSC implantation showed a positive role in promoting the recovery of the ulceration in foot with diabetic rats.

关 键 词：糖尿病足溃疡 人脐带间充质干细胞 表皮生长因子 

分 类 号：R587.2[医药卫生—内分泌]