氢气对大鼠肾脏缺血再灌注损伤的保护作用  

Protective Effects of Hydrogen on Renal Ischemia/Reperfusion Injury in Rats

作　　者：曾葵[1] 黄瀚[1] 江晓琴[1] 陈筱静[1] 黄蔚[1] 

机构地区：[1]四川大学华西第二医院麻醉科,成都610041

出　　处：《四川大学学报（医学版）》2014年第1期39-42,共4页Journal of Sichuan University(Medical Sciences)

基　　金：四川省科技计划项目(No.2009SZ0192)资助

摘　　要：目的探讨氢气对大鼠肾脏缺血再灌注损伤的保护作用及其机制。方法将18只成年雄性SD大鼠随机分成假手术(Sham)组、缺血再灌注(I/R)组、氢气治疗(H2)组3组,每组6只。I/R组大鼠右肾切除术后,制备左肾缺血再灌注损伤模型;Sham组大鼠右肾切除后,仅分离而不夹闭左肾肾蒂;H2组从再灌注前10min开始吸入浓度为2.5%的氢气130min,其余操作与I/R组相同。比较再灌注24h3组之间血清肌酐(Cr)、尿素氮(BUN),肾组织丙二醛(MDA)水平以及肾小管损伤的组织病理学半定量评分。结果 I/R可导致大鼠肾脏严重损伤。吸入浓度为2.5%的氢气可以降低大鼠BUN、Cr和MDA水平(P<0.05);同时,H2组肾组织病理学评分低于I/R组(P<0.05)。结论吸入低浓度的氢气对肾脏缺血再灌注损伤有保护作用,其机制与减少肾组织中MDA的生成有关。Objective To investigate the protective effects of hydrogen on renal ischemic/reperfusion injury and its mechanism in rats. Methods Eighteen adult male Sprague-Dawley rats were used and allocated into sham- operated group （Sham group）, ischemic reperfusion group C I/R group） and hydrogen treated group （ H2 group） randomly. Ischemic/reperfusion of the left renal pedicles following a right nephrectomy was investigated in I/R group. In the Sham group, the rats were subjected to identical surgical procedure without occlusion of left renal pedicles. In the H2 group？ inhalation of the 2. 5 % hydrogen was initiated 10 min before reperfusion and last for 130 min till the end of the 120-min reperfusion. Plasma level of usea nitrogen （BUN）,creatinine （Cr）,maiondiaidehyde （MDA）, the score of hivStopathological examination of left kidney were evaluated 24 h after operation in all subjects. Results Ischemia-reperfusion injury rat model was established successfully. Inhalation of hydrogen （ 2. 5 %） markedly reduced the level of serum BUN,CREA and MDA （P〈0.05）. The histopathological outcome in the H2 group was significantly improved,compared to the I/R group （P〈0. 05）. Conclusion Inhalation of hydrogen could attenuate renal ischemic/reperfusion injury in rats by decreasing the content of MDA.

关 键 词：肾脏 缺血再灌注损伤 大鼠 氢气 

分 类 号：R692[医药卫生—泌尿科学]