鹿茸血酒对去势大鼠的升雌激素与抗氧化作用的初步研究  

The Estrogen-like and Antioxidant Effects of Pilose Antler Blood Wine in Female Rats with Ovariectomy

作　　者：高强[1,2] 杨杨[1] 孙增春[1] 姜花[1] 贾雪[1] 袁菊莲[1] 何成奇[1,2,3] 

机构地区：[1]四川大学华西医院康复医学中心,成都610041 [2]四川大学-香港理工大学灾后重建与管理学院,成都610041 [3]康复医学四川省重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2014年第1期134-137,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.81171865);四川省科技厅(No.04JY029-087-3)资助

摘　　要：目的观察日常饮用鹿茸血酒对雌性去势大鼠的雌激素水平及抗氧化的影响。方法将60只大鼠随机分为6组,每组10只:正常对照组(不手术,每日管饲生理盐水9.1g/kg);去势对照组(卵巢切除术后,每日管饲生理盐水9.1g/kg);基酒对照组(卵巢切除术后,每日管饲基酒9.1g/kg);鹿茸血酒低、中、高剂量组(卵巢切除术后,每日管饲鹿茸血酒4.5g/kg、9.1g/kg、13.6g/kg),干预31d后,股动脉取血。采用黄嘌呤氧化法测定血清超氧化物歧化酶(SOD)的活性,采用硫代巴比妥酸法测定血清丙二醛(MDA)的含量,使用酶联免疫法测定血清雌二醇(E2)的水平。结果鹿茸血酒低剂量、中剂量和高剂量均可提高去势大鼠的血清E2(P<0.05)。鹿茸血酒低剂量和中剂量均可抑制去势大鼠血清SOD的下降,并且抑制MDA的上升(P<0.05);低剂量组抑制去势大鼠血清SOD下降和MDA上升的作用更为明显(P<0.05)。结论本研究条件下,日常饮用高剂量的鹿茸血酒31d可提高雌性去势大鼠的雌激素水平,低剂量的鹿茸血酒具有抗氧化作用。Objective To investigate whether pilose antler blood wine has the effects of estrogen-like activity and antioxidant in the ovariectomized （OVX） female rats. Methods The rat model of oxidative stress was established by ovariectomy. The female rats were divided into 6 groups： normal control group, OVX group, base wine group, and 3 pilose antler blood wine treated OVX groups （low, medium and high dose of wine at a dose of 4.5 g/kg, 9.1 g/kg and 13.6 g/kg respectively）. After the feeding of pilose antler blood wine （saline in control and OVX group） for 31 days, the serum estrodial level, serum superoxide dismutase （SOD）, and the content of methane dicarboxylic aldehyde （MDA） were measured. Results The one month treatment of daily drink of pilose antler blood wine could significantly promote the serum estrodial level in OVX rats （P〈0.05）. Either low or medium dose could inhibit the decrease of serum SOD activity and the increase of MDA content in OVX rats. and the inhibition effects were more obvious in low dose group compared with high dose group （P〈0.05）. Conclusion The daily drink of pilose antler blood wine showed the effects on increasing estrodial level and antioxidation in OVX rats.

关 键 词：鹿茸血酒 雌激素 抗氧化 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]