小肠黏膜下层与牙髓干细胞的生物相容性研究  

Biocompatibility of smaIl intestinal submucosa and human dental pulp stem cells in vitro

作　　者：张红梅[1] 侯莉娅 荀文兴[1] 王江[2] 李蓉[1] 曲晓莉[1] 曾光[1] 杨海珍[1] 

机构地区：[1]第四军医大学唐都医院口腔科,陕西西安710038 [2]第四军医大学西京医院神经外科,陕西西安710032

出　　处：《临床口腔医学杂志》2014年第1期9-12,共4页Journal of Clinical Stomatology

摘　　要：目的:观察小肠黏膜下层(small intestinal submucosa,SIS)对体外培养的人牙髓干细胞(human dental pulp stem cells,hDPSCs)生物学的影响及其作为支架材料对DPSCs牙向分化的诱导作用。方法:体外分离培养DPSCs,四唑盐比色法(MTS)比较观察SIS生物材料对DPSCs增殖活性的影响;茜素红染色、碱性磷酸酶(ALP)和逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)比较观察SIS生物材料对DPSCs牙向分化能力的影响。结果:SIS显著地刺激体外培养的DPSCs增殖,诱导了细胞的矿化和提高细胞ALP活性。RT-PCR结果显示SIS诱导后细胞mRNA水平表达牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)和牙本质基质蛋白(DMP-1)。结论:体外培养条件下,SIS与DPSCs有良好的生物相容性,作为支架材料对于DPSCs有较好牙向诱导作用。Objective：To observe the biological effects of small intestinal submucosa （SIS） on human dental pulp stem cells（DPSCs） cultured in vitro and investigate the odontogenic induction of DPSCs induced by SIS. Method： DPSCs were successfully isolated and cultured. The proliferation of DPSCs induced by SIS was evaluated by MTS assay. The odontogenic differentiation of DPSCs induced by SIS was measured using Alizarin red staining, the alkaline phosphatase （ALP） activity and reverse transcription-polymerase chain reaction （RT-PCR） assay. Result：The proliferation of DPSCs in SIS groups was obviously higher than that in the control groups. The higher levels of ALP activity and larger mineralizing nods were also detected in the experiment groups, RT-PCR demonstrated mRNA expression of dentin sialophosphoprotein （DSPP） and dentin matrix proteinl（DMP-1） in DPSCs induced by SIS. Conclusion：SIS possesses a good cellular compatibility with DPSCs. As an engineering scaffold, it can induce the odontogenic differentiation of DPSCs.

关 键 词：牙髓干细胞 小肠黏膜下层 增殖 分化 

分 类 号：Q813.1[生物学—生物工程]