MSCs及其上清液预防大鼠佐剂性关节炎的实验研究  

Research on the intervention of bone marrow MSCs and MSCs supernatant to adjuvant arthritis in rat

作　　者：许键炜[1] 何志旭[2] 申长清[3] 赵芳芳[4] 

机构地区：[1]贵阳医学院药理学教研室,贵阳550004 [2]贵阳医学院组织工程与干细胞实验中心,贵阳550004 [3]济宁医学院附属医院儿科,济宁272029 [4]蚌埠医学院微生物学教研室,蚌埠233030

出　　处：《安徽医科大学学报》2014年第1期22-26,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30560159);贵阳医学院基金(编号:K2009-37号)

摘　　要：目的探讨骨髓间充质干细胞(MSCs)及其上清液对大鼠佐剂性关节炎(AA)的预防作用。方法培养扩增SD大鼠骨髓MSCs,收集MSCs及其上清液。将健康雄性SD大鼠右后足跖皮内一次性注射弗氏完全佐剂0.1 ml/只造模。造模后行MSCs及其上清液干预。造模前1 d、造模后7、14、21、28 d测量大鼠体重和后足爪体积,同时观察大鼠生存状况。干预后第28天,行四肢关节X线片及病理检查。结果 MSCs干预组和上清液干预组大鼠,原发性炎症和继发病变均不明显,关节X线片和病理检查均未见明显异常,与模型组大鼠相比,体重和足爪体积差异有统计学意义(P<0.05)。结论 MSCs及其上清液可有效干预弗氏完全佐剂诱导的大鼠AA的进展。Objective To explore the effect of marrow mesenehymal stem cells （MSCs） and MSCs supernatant to prevent adjuvant arthritis （AA） in rats and its mechanisms. Methods MSCs which had been cultured and cloned with supematant would be collected. Rat＇ s right rear paw of the model group was injected intraeutaneously with 0. 1 ml of Freund＇ s complete adjuvant. After modeling, the rats were given MSCs and MSCs supernatant intervention. During the period, the rats＇ weights and hind paw volumes were measured on the 1st, 7th, 14th, 21st and 28th days respectively. And the rats＇ living conditions were observed. On the 28st day of the intervention, X-ray examination and pathological examination of rats＇ limb joints were carried out. Results After using MSCs supematant to intervene adjuvant arthritis, primary inflammation and secondary lesions were not obvious, and joint X-ray and pathological examination showed no obvious abnormalities. Compared with model group, the differences of rats＇ weight and paw volume had statistically significant （P 〈 0. 05 ）. Conclusion The results indicate that MSCs and MSCs supematant can intervene the progress of AA.

关 键 词：骨髓间充质干细胞 上清液 佐剂性关节炎 

分 类 号：R593.22[医药卫生—内科学]