初诊2型糖尿病患者呼出气冷凝液中肿瘤坏死因子α检测  

Detection of tumor necrosis factor-α in exhaled breath condensate in patients with newly diagnosed type 2 diabetes mellitus

作　　者：刘玉田[1,2] 陈金锋[1] 吴刚[1] 金艳[1] 王晓华[1] 徐峰[1] 苏建彬[1] 王雪琴[1] 

机构地区：[1]南通大学第二附属医院内分泌病科,江苏省226001 [2]南通大学医学院

出　　处：《江苏医药》2014年第1期58-60,共3页Jiangsu Medical Journal

摘　　要：目的探讨初诊2型糖尿病(T2DM)患者呼出气冷凝液中肿瘤坏死因子α(TNF-α)检测的可行性。方法用ELISA法检测32例初诊T2DM患者(T2DM组)和20例健康者(对照组)呼出气冷凝液及血清TNF-α水平,同时检测肺功能。结果 T2DM组呼出气冷凝液及血清TNF-α水平均高于对照组[(11.25±2.18)pg/ml vs.(5.36±1.27)pg/ml和(120.79±35.89)pg/ml vs.(27.23±7.02)pg/ml](P<0.05)。T2DM组肺功能相关指标均低于对照组(P<0.05)。T2DM组呼出气冷凝液及血清TNF-α水平与体重指数、糖化血红蛋白、胰岛素抵抗指数呈正相关(P<0.05),与肺一氧化碳弥散量呈负相关(P<0.05)。结论检测呼出气冷凝液中TNF-α水平有可能成为反映T2DM肺损害的新手段。Objective To investigate the feasibility of detecting tumor necrosis factor-α（TNF-α） in exhaled breath condensate （EBC） of patients with newly diagnosed type 2 diabetes mellitus （T2DM）. Methods With ELISA, the expressions of TNF-α in EBC and serum were detected in 32 patients with newly diagnosed T2DM（group A） and 20 healthy people（group C）. The lung functions were tested as well. Results The expressions o[ TNF-α in EBC and serum were higher in group A than those in group C[（11.25±2.18） pg/ml vs. （5.36± 1.27） pg/ml and （120.79±35.89） pg/ml vs. （27. 234±7.02） pg/ml] （P〈0. 05）. The lung function-related criteria were lower in group A than those in group C（P〈0.05）. The expressions of TNF-α in EBC and serum were positively correlated with body mass index, glycosylated hemoglobin Alc and insulin resistance index levels, but negatively correlated with carbon monoxide diffusion capacity（P〈0.05）. Conclusion The detection of TNF-α in EBC is hopefully to become a new potential approach for predicting diabetes lung injury.

关 键 词：2型糖尿病 肿瘤坏死因子Α 

分 类 号：R589[医药卫生—内分泌]