柑桔黄龙病常规PCR检测4对引物的特异性和灵敏度  

Comparison of the Specificity and Sensitivity of Four Pairs of Primer for PCR Detection of Candidatus Liberibacter asiaticus

作　　者：高玉霞 卢占军[1,2] 钟八莲 易龙[1,2] 赖特明 刘志芳[1] 肖珍 夏宜林 

机构地区：[1]赣南师范学院生命与环境科学学院,赣州341000 [2]国家脐橙工程技术研究中心

出　　处：《中国南方果树》2014年第1期5-8,共4页South China Fruits

基　　金：江西省科技计划项目(20111BBF60033);江西省科技计划项目(2009ZDN10100);江西省自然基金(20122BAB204024);赣州市农业公关项目;江西省青年基金(2011ZBAB214014)资助

摘　　要：通过荧光定量PCR标定柑桔黄龙病菌核酸浓度的方法,对目前各实验室常规PCR检测中应用较广泛的4对引物(P400f/P400r、P535f/P535r、OI1/OI2和16Sf/16Sr)进行特异性和灵敏度比较。结果表明,4对引物均能排除其他来源核酸的干扰,特异性均强;4对引物灵敏度大小关系为16Sf/16Sr>P400f/P400r>P535f/P535r=OI1/OI2,灵敏度最高的引物16Sf/16Sr可检测的柑桔黄龙病菌核酸浓度低限可达1.87×10-2ng/μL。Citrus Huanglongbing（HLB）is a dangerous plant disease in the world. In order to develop a quick, simple, sensitive and reliable method to detect HLB, four pairs of primer were compared by real time PCR in this study. The results showed that all these primer pairs P400f/P400t, P535f/ P535r, OI1/OI2 and 16Sf/16Sr were highly specific, but the order of sensitivity of these primer pairs was 16Sf/16Sr〉P400f/P400r 〉P535f/P535r=OI1/OI2. The primer 16SF/R was able to detect the HLB pathogen at nucleic acid concentration of 1.87×10^-2ng/μL.

关 键 词：柑桔黄龙病 常规PCR检测 引物 灵敏度 特异性 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]