一氧化氮在大鼠异种肝移植急性排异中的作用及其黄递酶组化研究  

The function of nitric oxide in acute rejection of golden hamster to rat orthotopic liver xenotransplantation and studies of NADPH-diaphorase histochemistry

作　　者：刁同进[1] 李又林[1] 赵晓东[1] 李冬梅[1] 姚晓平[2] 杨甲梅[2] 吴孟超[2] 

机构地区：[1]中国人民解放军第401医院肝胆外科,山东青岛266071 [2]中国人民解放军第二军医大学东方肝胆外科医院,上海200433

出　　处：《肝胆胰外科杂志》2000年第4期193-197,共5页Journal of Hepatopancreatobiliary Surgery

基　　金：国家自然科学基金资助

摘　　要：目的 :探讨一氧化氮 (NO)在肝移植急性反应中的功能作用及 NO合酶 (NOS)的细胞定位。方法 :应用金黄地鼠至大鼠异种原位肝移植急性排斥反应及大鼠同基因原位肝移植动物模型 ,观察 L - NMMA对受体存活期、AL T、TNF-α及移植肝的病理影响 ,应用 NADPH - d组化染色观察 NOS的活性及其细胞来源。结果 :表明 L - NMMA可明显降低异种肝移植组受体存活期、加剧其肝功能恶化、上调 TNF-α、加剧移植肝的病理损害 ;异种肝移植组 NADPH- d组化染色呈强阳性 ,主要在肝实质细胞及浸润炎性细胞表达。结论Objective:To investigate the role of nitric oxide in acute rejection of golden hamster to rat orthotopic liver xenotransplantation and the cellular origin of nitric oxide synthase. Methods:By establishing the model of golden hamster to rat orthotopic liver xenotransplantation following acute rejection and Wistar rat to rat syngeneic orthotopic liver transplantation,we observed the effect of NO synthase inhibitor(L NMMA) on the recipient survival,ALT,TNF α and pathology of grafted liver. Using NADPH diaphorase histochemistry staining,we studied the activities of NO synthase and cellular origin of nitric oxide. Results:Administration of NO synthase inhibitor(L NMMA) can markedly reduce the recipient survival,deteriorate the grafted liver function,upregulate the expression of TNF α,gravitated the pathological injury of the grafted liver. The expression of nitric oxide synthase was primarily present in both hepatocytes and portal inflammatory infiltrate cells for NADPH diaphorase histochemistry staining in orthotopic liver xenotransplantation.Conclusion:These results suggest that nitric oxide may play a potential immunologic protective role within the settings of acute rejection of golden hamster to rat orthtopic liver xenotransplantation.

关 键 词：肝移植 急性排斥反应/外科学 一氧化氮 一氧化氮合酶/代谢 大鼠 金黄地鼠 NADPH-d黄递酶组化 

分 类 号：R657.3[医药卫生—外科学]