六君子汤合左金丸治疗小鼠急性放射性十二指肠炎的疗效及机制研究  

Study on efficacy of Liujunzi decoction combined with Zuojin pills in treating acute radioactive duodenitis and their mechanism

作　　者：陈晓[1] 万芝清[1] 韩根成[2] 王济东[1] 赵智[2] 周平[1] 

机构地区：[1]解放军空军总医院,北京100142 [2]军事医学科学院基础医学研究所分子免疫室,北京100850

出　　处：《中国中药杂志》2014年第2期278-284,共7页China Journal of Chinese Materia Medica

基　　金：首都卫生发展专项(2011-5041-04)

摘　　要：目的:评估六君子汤合左金丸治疗小鼠急性放射性十二指肠炎的疗效,与临床常规抑酸联合黏膜保护制剂对比,探讨其产生疗效的机制。方法:根据文献研究,参考放射性肠炎模型建模标准结合十二指肠特征,首创小鼠放射性十二指肠损伤模型。将模型小鼠随机分为对照组(n=26)、中药干预组(n=16)、西药干预组(每只法莫替丁悬液0.5 mL+铝镁加混悬液0.3 mL灌胃,每日1次,n=16)。标准给药后通过小鼠一般情况、体重、健康评分变化、病理学、炎性因子表达等客观指标评价疗效。结果:选取12 Gy成功建立小鼠放射性十二指肠炎模型。对照组小鼠出现体重下降,厌食、少饮、稀便,偶有黏液血便,精神差,皮毛不光整、少动、弓背等表现。药物干预组小鼠一般情况较单纯照射组好转。中、西药干预组脾、肠系膜淋巴结组织IL-6,IL-1β,TNF-α的表达与对照组相较有下降趋势,外周血中三者浓度变化亦有所体现,中药组在降低炎性因子方面优势显著。镜下观察可见中、西药干预组较单纯照射组黏膜有所修复,改良Chiu氏法评分中西药组间差异有统计学意义(P<0.05)。结论:六君子汤合左金丸治疗急性放射性十二指肠炎除能调节机体免疫、抑制急性损伤、促进局部组织修复外,还具有抗辐射肠道纤维化等不良反应潜力,其中调节炎性因子释放是疗效产生机制之一。Objective： To evaluate the therapeutic effect of Liujunzi decoction combined with Zuojin pills in treating the radio- active duodenitis and their mechanism, and compare with clinical routine acid suppressants combined with mucous membrane protective preparations to study the mechanism of their efficacy. Method： According to the study of Williams J P and characteristics of duodeni- tis, and by reference to the radiation enteritis modeling standard, we took the lead in establishing the mouse radioactive duodenal injury model The model mice were randomly divided into the control group （n = 26）, traditional Chinese medicine（TCM） group （n = 16） and the western medicine （ oral administration with famotidine 0. 5 mL ＋ almagate suspension 0. 3 mL per mouse, once a day） group （n = 16）. After the standard administrating, such objective indexes as general condition, weight, changes in health score, pathology and expression of inflammatory factors were observed to evaluate the efficacy. Result： The radioactive duodenitis model of mice was successfully established with 12 Gy. Mice in the control group suffered from weight loss, anorexia, low fluid intake, loose stools, and occasionally mucous bloody stool, poor spirit, dim fur, lack of exercise and arch back. Mice in drug intervention groups were generally better than those in the pure irradiation group. The IL-6, IL-1β, TNF-α mRNA expressions in spleen and mesenterie lymph node tis- sues in TCM and western medicine groups showed a declining trend compared with the control group. Their concentrations in peripheral blood serum also slightly changed. The TCM group revealed notable advantage in reducing inflammatory factors. The microscopic obser- vation showed that a better mucosa repair in intervention groups than the pure irradiation group. The improved Chiu＇s scoring method showed a statistical significance in the difference between TCM and western medicine groups （P 〈 0. 05）. Conclusion： Liujunzi decoc- tion combined with Zuojin pills

关 键 词：六君子汤 左金丸 急性放射性肠炎 

分 类 号：R259[医药卫生—中西医结合]