铁皮石斛多糖促进毛发生长的实验研究  

Experimental study on Dendrobium candidum polysaccharides on promotion of hair growth

作　　者：陈健[1] 戚辉[1] 李金标[1] 易燕群[1] 陈丹[1] 胡小红[1] 王美玲[1] 孙兴力[2] 魏小勇[1] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]永州职业技术学院药学系,湖南永州425100

出　　处：《中国中药杂志》2014年第2期291-295,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81102674;81274157;30850012);广东省自然科学基金项目(S2011010005661;06024128);广东省科技计划项目(2011B031700076;2009B060700070;2007B020704005;2009A030100003;2009B090300335)

摘　　要：目的:观察铁皮石斛多糖(DCP)促毛发生长的功效并探讨其作用机制,为铁皮石斛的开发利用奠定基础。方法:采用水提醇沉法提取DCP,并用苯酚-硫酸法测定其多糖含量。取C57BL/6J小鼠30只,用脱毛膏去除其背部毛发建立脱发模型,分为对照组、阳性对照组和DCP组,每日分别涂抹超纯水、米诺地尔酊、DCP(5.0 g·L-1)l次,每次0.2 mL,连续给药21 d,应用小鼠毛发生长情况评分标准对C57BL/6J小鼠给药7,14 d毛发生长情况进行评分,及称量给药21 d C57BL/6J小鼠单位脱毛区域毛发质量评价DCP对C57BL/6J小鼠毛发生长的影响。采用MTT法和RT-PCR法分别评价DCP对人永生化角质形成细胞(HaCaT细胞)增殖和对HaCaT细胞血管内皮生长因子(VEGF)mRNA表达的影响。结果:DCP提取率为29.87%,DCP质量分数为79.65%。DCP组C57BL/6J小鼠的毛发生长平均得分、平均质量均显著优于对照组;DCP组HaCaT细胞生存率和VEGF mRNA的表达水平与对照组比较均显著提高。结论:DCP具有促毛发生长的作用,其机制可能是上调VEGF mRNA的表达。Objective： To observe the effect and mechanism of Dendrobium candidum polysaccharides （DCP） in promoting hair growth, in order to lay a foundation for the development and utilization of D. candidum. Method： The water-extraction and alco- hol-precipitation method was adopted to extract DCP, and the phenol-sulphuric acid method was used to determine its content. Thirty C57BL/6J mice were collected to establish the hair loss model with hair removal cream. They were randomly divided into the control group, the positive control group and the DCP group, and given 0. 2 mL of ultra-pure water, minoxidil tincture and DCP （5.0 g · L^- 1 ） 21 days. The mice hair growth scoring standard was adopted to evaluate the hair growth of C57BL/6J mice at 7, 14 d. The hairs in unit hair-losing areas of treated C57BL/6J mice at 21 d were weighed to evaluate the effect of DCP on the promotion of hair growth. MTT as- say and RT-PCR method were used to evaluate the effect of DCP on the proliferatin of HaCaT ceils and the mRNA expression of VEGF in HaCaT cells. Result： The extraction percent of DCP was 29. 87%, and its content was 79. 65%. The average scores for the hair growth and weight of C57BL/6J mice of DCP group were much higher than the control group. The survival rate and mRNA expression of VEGF of HaCaT cells were much higher than the control group. Conclusion： DCP has the effect in promoting hair growth. Its mech- anism may be related to the up-regulation of the mRNA expression of VEGF.

关 键 词：铁皮石斛多糖(DCP) 毛发生长 血管内皮细胞生长因子(VEGF) 

分 类 号：R285.5[医药卫生—中药学]