鱼腥草素钠增强亚胺培南抗铜绿假单胞菌生物被膜的作用  

Effect of sodium houttuyfonate in enhancing imipenem's activity against Pseudomonas aeruginosa biofilms

作　　者：程惠娟[1] 杨敏[1] 汪长中[1] 王艳[1] 张昌峰[1] 褚春云 

机构地区：[1]安徽中医药大学,安徽合肥230038

出　　处：《中国中药杂志》2014年第2期296-299,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173629)

摘　　要：目的:研究鱼腥草素钠联合亚胺培南增强抗铜绿假单胞菌生物被膜的作用。方法:2倍稀释法测定被试药物最小抑菌浓度(MIC),结晶紫染色法检测1/2MIC,1MIC,2MIC的鱼腥草素钠(SH)、亚胺培南(IMP)单药组和1/2MIC的SH与IMP各药物浓度联合组对黏附菌的清除量、生物被膜生长量、藻酸盐产生量的影响。FDA-PI荧光素双染法荧光显微镜下观察各药物组膜分散后死活细菌、生物被膜形态。结果:鱼腥草素钠能增强亚胺培南抗绿假单胞菌生物被膜的作用,尤以2MIC药物浓度联合组作用最强(与阴性对照组比较P<0.001)。荧光显微镜下观察死活细菌、生物被膜形态支持了上述结果。结论:鱼腥草素钠联合亚胺培南能增强抗生物被膜作用,预期两药联合会提高相关感染的临床疗效。Objective： To investigate the resistant effect of houttuyfonate sodium （ SH ） combined with imipenem （IMP） against Pseudomonas aeruginosa （Pa） biofilms. Method： The two-fold dilution method was used to examine the minimum inhibitory concentration （MIC） of the tested drug. The crystal violet staining was applied to detect the effect of the combination of 1/2MIC, 1MIC, 2MIC of SH, single IMP, 1/2MIC of SH and IMP of various concentrations on the clearance rate of adherent bacteria, growth of biofilms and alginate production. Fluorescein diacetate （FDA）-propidium iodide （PT） doubling staining assay was employed to observe the bacterial viability and morphological changes after membrane dispersion of each drug group. Result： Sodium houttuyfonate could enhance the effect of IMP against pseudomonas aeruginosa biofilms. Particularly, the combination group with the concentration of 2MIC showed the highest effect, with P 〈0. 001 compared with the negative control group. The above results were proved by the bacterial via- bility and biofilm morphology under fluorescence microscope. Conclusion： After being combined with imipenem, sodium houttuyfonate shows a higher effect against biofilms. It is expected that the combination of the two drugs could improve the clinical efficacy of associ- ated infections.

关 键 词：铜绿假单胞菌 生物被膜 鱼腥草素钠 亚胺培南 最小抑菌浓度 

分 类 号：R96[医药卫生—药理学]