大黄中游离蒽醌类化合物在实验性急性胰腺炎大鼠的组织药理学研究  

Pharmacological study on free anthraquinones compounds in rhubarb in rats with experimental acute pancreatitis

作　　者：竹林[1] 赵健蕾[1] 彭小航[1] 万美华[1] 黄熙[2] 唐文富[1] 

机构地区：[1]四川大学华西医院中西医结合科,四川成都610041 [2]中南大学湘雅医院中西医结合科,湖南长沙410005

出　　处：《中国中药杂志》2014年第2期304-308,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81001511)

摘　　要：目的:研究熟大黄主要成分在急性胰腺炎大鼠模型体内靶向分布情况,以验证方剂组织药理学假说。方法:采用正常SD大鼠(正常组,n=5)、蛙皮素腹腔注射建立急性胰腺炎模型大鼠(模型组,n=5)作为实验对象。熟大黄颗粒剂(20g·kg-1)每周灌胃2组大鼠1次,2 h后取心、肝、脾、肺、肾、胰腺等6个组织,运用高效液相色谱-质谱法测定各组织匀浆中熟大黄中大黄素、大黄酚、大黄素甲醚、大黄酸、芦荟大黄素等成分谱及其浓度。结果:除熟大黄的芦荟大黄素和大黄素甲醚在正常大鼠肝脏和肾脏内的浓度高于胰腺组织内的浓度之外,胰腺组织内各个成分的分布谱及浓度并与其他的器官没有明显差异;熟大黄中的大黄素、芦荟大黄素、大黄酸和大黄酚在急性胰腺炎模型大鼠胰腺组织中的浓度高于其他组织器官,且在胰腺、肾、脾组织内的浓度明显高于正常组。结论:在急性胰腺炎的状态下,熟大黄的有效成分在胰腺具有靶向分布特点,为方剂组织药理学假说提供了实验依据。Objective： To verify the pharmacological hypothesis of prescriptions by studying the targeted distribution of major components in stewed rhubarb in the rat model with acute pancreatitis （AP）. Method： Normal SD rats （control group, n = 5 ） and the AP model induced with intraperitoneal cerulein （model group, n = 5 ） were taken as the experimenta! objects. Rats of the two groups were orally administered with stewed rhubarb granules （20 g · kg^-1 ）. Their heart, liver, spleen, lung, kidney and pancreas were col- lected two hours after the administration. Such constituents as emodin, chrysophanol, physcion, rhein and aloe-emodin and their con- centrations in each tissue homogenate were detected by high performance liquid chromatography-mass-mass. Result： Aloe-emodin and physcion in stewed rhubarb whose concentrations in liver and kidney of normal rats were higher than that in pancreatic tissues, while the distribution spectrums and concentrations of the remaining components in pancreatic tissues had no significant difference with that of other organs. The concentrations of emodin, aloe-emodin, rhein and chrysophanol in stewed rhubarb in pancreatic tissues of the AP model group were higher than that in other tissues and organs, while their concentrations in pancreatic, renal and splenic tissues were notably higher than that in the normal group. Conclusion： In the conditions of AP, effective components in stewed rhubarb show a tar- geted distribution feature in pancreas, which provides experimental basis for the pharmacological hypothesis of prescriptions.

关 键 词：急性胰腺炎 熟大黄 有效成分 方剂组织药理学 靶向分布 

分 类 号：R285.5[医药卫生—中药学]