钙拮抗氟对大鼠睾丸组织的氧化损伤作用  

Antagonistic Effect of Calcium against Fluorosis Induced Oxidative Stress in Rat Testis

作　　者：吴秋云[1] 许爱芹[1] 杨向东[1] 王锋[1] 

机构地区：[1]徐州医学院公共卫生学院,江苏221004

出　　处：《环境与职业医学》2014年第1期52-54,共3页Journal of Environmental and Occupational Medicine

基　　金：2011年徐州医学院科研课题;2011年徐州医学院公共卫生学院科研课题

摘　　要：[目的]研究氟对大鼠睾丸组织过氧化氢酶(CAT)、乳酸脱氢酶(LDH)和丙二醛(MDA)水平的影响以及钙对其拮抗作用。[方法]选用健康雄性清洁级SD大鼠80只,随机分为10组:对照组、加钙染氟6个剂量组[NaF 2.1、4.2、8.5、17.0、34.0、68.0 mg/(kg·d);CaCO325、50、50、100、100、150 mg/(kg·d)]和不加钙染氟3个剂量组[NaF2.1、8.5、34.0 mg/(kg·d)],灌胃染毒90 d。染毒结束次日,观察大鼠精子数量、睾丸组织结构的改变,并测定睾丸组织中CAT、LDH和MDA水平。[结果]氟可使大鼠睾丸精子计数下降并导致睾丸组织病理学改变。各组大鼠睾丸组织CAT、LDH和MDA含量组间比较,差异有统计学意义(F值分别为5.168、2.248、3.168,P值分别为<0.001、0.044、0.007)。高剂量染氟组CAT[(27.86±4.04)U/mg prot]较对照组[(44.85±2.81)U/mg prot]降低、LDH[(38 358.49±2 649.23)U/g prot]较对照组[(54 357.66±1 903.90)U/g prot]降低、MDA[(6.82±0.79)nmol/mg prot]较对照组[(3.59±1.11)nmol/mg prot]升高。在相同染氟浓度下,适量加钙组较不加钙组CAT水平升高、MDA水平降低。[结论]氟引起睾丸组织的损害可能与氧化应激作用有关,适量的钙可以拮抗氟诱导的自由基水平增高所造成的氧化损伤作用。[Objective] To investigate the effects of fluoride on catalase （CAT） , lactate dehydrogenase （LDH） , and malonic dialdehyde （MDA） in rat testis and the potential antagonism against these effects by calcium. [Methods] Eighty healthy male SD rats were equally randomized into 10 groups： 1 control group, 6 fluorosis groups with calcium （NaF： 2.1, 4.2, 8.5, 17.0, 34.0, 68.0 mg/kg·d;CaCO3：25, 50, 50, 100, 100, 150 mg/kg·d, respectively）, and 3 fluorosis groups without calcium （NaF： 2.1, 8.5, 34.0 mg/kg·d） . The exposure by intragastric administration lasted 90 days. After the last dosing, sperm count, pathological alternations, and levels of CAT, LDH, and MDA in testis samples were determined. [Results] The fluoride exposure reduced the sperm count and damaged the structure of testis in tested rats. The differences in CAT, LDH, and MDA levels among the experiment groups were statistically significant （F was 5.168, 2.248, 3.168;P 〈 0.001, 0.044, 0.007, respectively）. Compared with the control group, in high-dose fluorosis group treated with 34 mg/kg·d NaF without CaCO3, the level of CAT decreased [（27.86±4.04） U/mg prot vs （44.85±2.81） U/mg prot], the level of LDH also decreased [（38 358.49±2 649.23） U/g prot vs （54 357.66±1 903.90） U/g prot], while the level of MDA increased [（6.82±0.79） nmol/mg prot vs （3.59±1.11） nmol/mg prot]. Higher level of CAT and lower level of MDA were observed in the group treated with calcium presented than those in the one with the same dose of fluoride but without calcium. [Conclusion] Fluoride can affect the testis tissue in rats by oxidative stress. The oxidative damage through increasing free radical induced by fluoride may be antagonized by appropriate dose of calcium.

关 键 词：氟 氧化应激 钙 拮抗作用 睾丸 

分 类 号：R114[医药卫生—卫生毒理学]