鸡传染性贫血病毒广东分离株GD-1-12致病性研究  

Study on Pathogenicity of a Guangdong Strain GD-1-12 of Chicken Anemia Virus

作　　者：张新珩 刘远佳[2] 吴波良 陈峰[1] 孙宝丽[1] 马静云[1] 谢青梅[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]华南农业大学兽医学院,广东广州510642

出　　处：《动物医学进展》2014年第1期27-31,共5页Progress In Veterinary Medicine

基　　金：广东省重大科技项目(2009B020201008)

摘　　要：从广东省某商品代肉鸡场12日龄生长迟缓的小鸡体内分离到1株鸡传染性贫血病毒（CAV），暂定名为GI）_1—12株。分离病毒用MDCC—MSB1细胞进行培养，通过肌肉注射方式感染1日龄SPF鸡，于感染后第7天、第14天、第21天分别检测CAV对SPF鸡体质量、胸腺、脾脏、肝脏、法氏囊及胸腺和外周血液中CD3＋T淋巴细胞指标的影响，同时观察鸡的临床症状。结果表明，GD-1—12分离株导致SPF鸡30％的死亡率。感染后第14天，胸腺极度萎缩，肝脏肿大，法氏囊萎缩，脾脏萎缩。感染后第7～14天之间，胸腺和外周血中CD3＋T淋巴细胞呈下降趋势。结果表明1日龄SPF雏鸡在人工感染CAV后第7～14天是损伤上述器官的关键时期。A field strain GD-l-12 of chicken anemia virus （CAV） was isolated from a 12-day-old broiler flock with growth runting syndromes from Gongdong province. MDCC-MSB1 cells were used to proliferate the virus of GD-l-12 isolate. SPF chickens at 1 day were challenged with the virus by intramuscular injection. Ratios of thymuses, spleens, livers, the bursas to body weight, and the CD3＋ T cells in thymuses and pe- ripheral blood were analyzed after infection at 7, 14, 21-day. Observation of clinical symptoms at 7, 14, 21-day after infections with CAV. Results indicated that the GD-I-12 isolate caused high mortality about 30%, belonging to highly pathogenic. After infection at 14 day, resulting in severe thymic atrophy, hepa- tomegaly, bursa atrophy, spleen atrophy. After challenging at 7-14 day, the CD3＋ T cells in thymuses and peripheral blood were significantly decreased, it indicated that T lymphocytes were the target cells. The research further illustrated that after infection at 7-14 day was the critical period of the immune func- tion with the serious obstacle.

关 键 词：鸡传染性贫血病毒 SPF鸡 致病性 

分 类 号：S852.659.2[农业科学—基础兽医学] S858.31[农业科学—兽医学]