小白菊内酯对人乳腺癌细胞株增殖敏感性的影响  

Inhibition effect of parthenolide on the human breast cancer BT549 cells

作　　者：徐惠君[1] 贾勇圣[1] 陈悦[2] 佟仲生[1] 

机构地区：[1]天津医科大学肿瘤医院乳腺内科,国家肿瘤临床医学研究中心,乳腺癌防治教育部重点实验室,天津市肿瘤防治重点实验室,天津300060 [2]南开大学药学院,天津300071

出　　处：《天津医科大学学报》2014年第1期11-13,共3页Journal of Tianjin Medical University

基　　金：天津市重大课题专项基金资助项目(12ZCDZSY16200)

摘　　要：目的:观察小白菊内酯(PN)单独及联合顺铂(DDP)对人乳腺癌细胞株BT549生长的影响,并探讨其作用机制。方法:体外培养人乳腺癌BT549细胞株,分别加入不同浓度的PN、DDP及PN+DDP至细胞中,作用48 h后,分别采用CCK8细胞增殖实验检测细胞存活率,流式细胞仪检测细胞周期。结果:随着PN工作液浓度的升高,BT549细胞48 h的细胞存活率逐渐降低。同样,随着DDP工作浓度的升高,BT549细胞48 h的细胞存活率也逐渐下降。并且相应浓度的PN和DDP联合作用于BT549细胞48 h的存活率降低更加明显,且差异具有统计学意义(P<0.05)。流式细胞仪测细胞周期实验发现,这两种药物主要通过阻滞细胞周期G1/S期的转化。结论:PN单用及联合DDP作用均可抑制BT549细胞的增殖,且呈浓度-剂量依赖性,两药联合应用对细胞增殖的抑制作用更加明显,表明这两种药物具有协同作用。Objective： To observe the effect of parthenolide （PN） alone and in combination with cis-platinum （DDP） on the growth of human breast cancer cells BT549 and explore its mechanism. Methods：Human breast cancer BT549 cells were cultured in vitro. Different concentrations of PN, DDP and PN combined with DDP were added to BT549 cells. After 48 h, the cell viability rate was detected by CCK8 assay. Cell cycle distribution was tested by flow cell cycle experiment. Results：The cell viability rate declined after different concentrations of PN were added to BT549 cells in 48 h. And the cell viability rate decreased after different concentrations of DDP were added to BT549 cells in 48 h, and the difference was statistically significant （P〈0.05）. The cell viability rate sharply reduced after corresponding concentrations of PN combined with DDP were added to BT549 cells in 48 h. Cell cycle G1/S transition was mainly blocked by the two drugs. Conclusion：PN alone and in combination with DDP can both inhibit the proliferation of BTB49 cells in concentrationdose dependant. And the drugs also show synergistic effect.

关 键 词：小白菊内酯 顺铂 乳腺癌细胞 细胞周期 

分 类 号：R969[医药卫生—药理学]