胸膜斑阳性接尘工人血清效应蛋白的研究  

A study of serum proteins in asbestos-exposed workers with pleural plague

作　　者：鞠莉[1] 陈钧强[1] 朱丽瑾[1] 张敏[1] 贾振宇[1] 张幸[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《浙江预防医学》2014年第1期10-13,18,共5页Zhejiang Journal of Preventive Medicine

基　　金：浙江省卫生厅优秀青年基金(2008QN002);浙江省医学重点学科--支撑学科(11-ZC02)

摘　　要：目的监测胸膜斑阳性接尘工人血清效应蛋白质,探讨胸膜斑发病机制及其与石棉肺的关系。方法将研究对象分为胸膜斑组、阴性对照组和正常对照组。各组人群静脉血应用双向电泳和质谱进行分离和鉴定,以灰度值差异1.5倍为基准获得差异表达蛋白质。结果胸膜斑组与阴性对照组和正常对照组比较,差异点分别有34和16个;阴性对照组与正常对照组比较,差异点有32个。效应蛋白质如补体因子、α1-抗胰蛋白酶等参与机体免疫、抗肿瘤等过程。部分效应蛋白质与石棉肺血清效应蛋白一致。结论长期接触石棉导致血清中某些蛋白质表达量改变,进而影响其生物学功能。胸膜斑和石棉肺有相同的靶效应蛋白,提示胸膜斑工人更易患石棉肺。Objective To separate serum proteome of workers with pleural plague for further understanding of the mechanisms of pleural plague, and to explore its potential relationship with asbestosis. Methods Workers were assigned into three groups： pleural plague group, negative control group and normal control group, then a proteomic analysis was used to screen and identify the differentially expressed serum proteins of all workers. ImageMaster6.0 software was used to analyze the differentially expressed proteins and a 1.5 fold change was considered significant. Results There were 34 and 16 spots differentially expressed in pleural plague group compared with negative control group and normal control group respectively. While there were 32 spots differentially expressed between negative control group and normal control group. The proteins were identified as CA, cytokines, α1 - AT, etc. Some proteins identified were the same as that screened in serum of workers with asbestosis. Conclusion Expression changes of some serum proteins are found in workers with pleural plague, which may further influence normal biological functions. Some proteins are found both in pleural plague serum and asbestosis serum which leaves a hint that workers with pleural plague have higher risks of getting asbestosis.

关 键 词：石棉 胸膜斑 血清 蛋白质组 石棉肺 

分 类 号：R135.2[医药卫生—劳动卫生]