针刺对抑郁症大鼠海马CREB mRNA、BDNF mRNA表达的影响  

Effect of Acupuncture on Cyclic Adenosine Monophosphate Response Element Binding Protein and Brain-derived Neurotrophic Factor mRNA Expression in Hippocampus of Depression Rats

作　　者：孙健[1] 张继福[1] 符文彬[1] 刘健华[1] 樊凌[1] 

机构地区：[1]广东省中医院针灸科,广东广州510120

出　　处：《广州中医药大学学报》2014年第1期62-65,71,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然基金项目资助(编号:30772828;81173348);广东省自然科学基金项目资助(编号:07004846);教育部高校博士点专项科研基金资助(编号:20094425110005)

摘　　要：【目的】探讨针刺对抑郁症大鼠海马cAMP反应结合蛋白(CREB)、脑源性神经营养因子(BDNF)mRNA表达的影响。【方法】选用SD成年大鼠32只随机分为空白组、模型组、针刺组和西药组(盐酸氟西汀,剂量为1.8 mg·kg-1·d-1),每组8只。空白组进行正常饲养,不给予其他处理;模型组大鼠采用长期不可预见性温和刺激法复制抑郁症模型;针刺组大鼠造模后针刺合谷穴和太冲穴,并加电针,每日1次,每次15 min,左右两侧穴位交替进行,持续21 d。西药组大鼠造模后以氟西汀灌胃,每日1次,持续3周。应激后22 d以颈椎脱臼法处死大鼠,取大鼠海马组织存储于-70℃备用,采用实时荧光定量PCR方法检测海马CREB mRNA、BDNF mRNA的表达。【结果】抑郁症模型组大鼠海马中的CREB mRNA、BDNF mRNA表达较空白组显著下降(P<0.01);西药组和针刺组大鼠海马CREB mRNA表达较模型组显著升高(P<0.05);西药组和针刺组大鼠海马BDNF mRNA表达与模型组比较,差异无统计学意义(P>0.05)。【结论】针刺能改善抑郁症大鼠海马CREB mRNA表达,这可能是针刺抗抑郁作用的机理之一。Objective To investigate the effect of acupuncture on cyclic adenosine monophosphate （cAMP） response element binding protein （CREB） and brain-derived neurotrophic factor （BDNF） mRNA expression in the hippocampus of depression rats. Methods Thirty-two adult Sprague-Dawley rats were randomly divided into 4 groups, blank group, model group, medicine group and acupuncture group, 8 rats in each group. Rats in the blank group were fed by the conventional method, and the rats in the other three groups were given chronic unpredictable mild stress to establish depression model. After modeling, the acupuncture group was given electroacupuncture on unilateral Hegu and Taichong acupionts alternatively for 15 minutes, once daily, for 21 continuous days; the medicine group was treated with fluoxetine hydrochloride, once per day for 3 weeks. On the twenty-second day after experiment, all the rats were executed by cervical dislocation, and then the hippocampus tissue was taken out and reserved in the refrigerator at -70℃. CREB and BDNF mRNA expressions in the hippocampus were detected by fluorescence real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR）. Results The expression levels of CREB and BDNF mRNA in rats hippocampus of the model group were significantly decreased （P〈O.O1 compared with those of the blank group）. CREB mRNA expression in rats hippocampus of the medicine group and the acupuncture group was significantly increased （P〈0.05 compared with that of the model group）, while BDNF mRNA expression in rats hippocampus of the both groups had no significant difference from that of the model group （P〉0.05）. Conclusion One of the mechanisms of acupuncture for relieving depression is probably related with the improvement of CREB mRNA expression in the hippocampus of depression rats.

关 键 词：抑郁症 针灸疗法 基因表达调控 疾病模型 动物 大鼠 

分 类 号：R245.31[医药卫生—针灸推拿学]