慢性HBV感染者血清中人MASP水平检测的临床意义  

The clinic significance of serum MASP detection in HBV chronic infected patients

作　　者：蒋静[1] 王拥军[2] 薛黎[1] 何超[3] 

机构地区：[1]新疆医科大学第一附属医院检验科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院输血科,新疆乌鲁木齐830054 [3]兰州大学第一医院医学检验中心,甘肃兰州730000

出　　处：《国际检验医学杂志》2014年第1期9-11,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(编号:30660718);新疆维吾尔自治区病原生物学重点学科资助项目(编号:BYX11001)

摘　　要：目的研究甘露糖结合凝集素相关的丝氨酸蛋白酶(MASP)在病毒不同感染程度的乙肝患者中的表达及其临床意义。方法乙肝患者共140例,根据血清中丙氨酸转氨酶(ALT)和HBeAg的水平分为6组:A组(ALT正常,HBeAg阳性)、B组(ALT正常,HBeAg阴性)、C组(ALT轻度/中度升高,HBeAg阳性)、D组(ALT高度/重度升高,HBeAg阳性)、E组(ALT轻度/中度升高,HBeAg阴性)和F组(ALT高度/重度升高,HBeAg阴性)。采用ELISA检测甘露聚糖结合凝集素相关丝氨酸蛋白酶水平在健康对照组(即G组)及患者血清中的水平,并与ALT、AST、HBSAg、HBeAg、HBV-DNA作相关性分析。结果 F组的MASP水平显著高于A组、C组和D组,同时其显著低于B组和G组(P<0.05);D组的MASP水平显著低于其余6组(P<0.05);G组的MASP水平显著高于其余6组(P<0.05);MASP水平与ALT、AST、HBSAg、HBeAg、HBV-DNA的值存在相关性(P<0.05)。结论乙肝患者血清中MASP水平会降低,且其水平异常降低与患者肝损伤程度加重、病毒感染和复制水平加剧均有关系。Objective To research the expression and clinical signifance of MASP in patients with different gradient of HBV in- fection. Methods The 140 patients were divided into six groups by its HbeAg expression and ALT level.. Group A （HBeAg posi- tive, ALT normal）, group B （ HBeAg-negative, ALT normal）, Group C （ HBeAg positive, ALT mildly / moderately elevated）, group D （HBeAg positive,ALT highly / severely increased）, group E （HBeAg negative, ALT mildly / moderately elevated）, group F （HBeAg negative, ALT highly / severely increased） , and group G was the normal control group. ELISA was used to test the level of c3b in serum of each samples, and the correlation analysis was done with ALT, AST, HBSAg, HBeAg and HBV DNA. Results The MASP level of F group was significantly higher than that of group A,C and D （P〈0.05） ,and the MASP level of group D was significantly lower lhan that of the other groups （P〈0.05）. The MASP level of G group was significantly higher than that of the other groups （P〉0.05）, and the level of MASP was positively correlated with ALT, AST, HbsAg and HBV-DNA （P〈0.05）. Conclusion The serum MASP levels of HBV infected patients are low. The abnormal low level of MASP can be assisted detection index of increase infection and prognostic.

关 键 词：甘露糖结合外源凝集素 丝氨酸内肽酶类 肝炎病毒 乙型 丙氨酸转氨酶 

分 类 号：R512.62[医药卫生—内科学]