Elecsys HBsAg Ⅱ确证试验检测临界及弱反应性标本的临床意义  

The clinical significance of the HBsAg Ⅱ confirmatory analysis in detection of HBsAg cutoff and weakly reactive samples

作　　者：姚家奎[1] 孙蓉[1] 周林[1] 张素华[1] 成红霞[1] 

机构地区：[1]江苏省苏北人民医院临床医学检测中心,江苏扬州225001

出　　处：《国际检验医学杂志》2014年第1期47-48,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨Elecsys HBsAgⅡ确证试验在乙肝两对半定量不同模式间HBsAg处于临界及弱反应性标本检测中的临床意义。方法所有被检血清标本均来自该院2011年3月至2013年8月就诊的门诊和住院患者,对128例不同模式两对半定量结果HBsAg处于临界及弱反应标本进行了Elecsys HBsAg确证试验及结果对比分析。结果 HBsAg处于临界及弱反应标本检出的乙肝两对半模式有6种分别为模式一(HBsAg、HBeAb、HBcAb结果有反应性)58例;模式二(HBsAg、HBsAb、HBeAb、HBcAb有反应性)11例;模式三(HBsAg、HBsAb、HBcAb结果有反应性)8例;模式四(HBsAg、HBcAb结果有反应性)14例;模式五(HBeAb、HBcAb结果有反应性)23例;模式六(HBsAb、HBcAb结果有反应性)14例。各种模式HBsAg定量分析COI均值分别为1.97、2.15、2.05、2.17、0.89、0.88;与其确证试验结果相符率分别为77.59%(45/58)、81.82%(9/11)、100.00%(8/8)、71.43%(10/14)、100.00%(23/23)和100.00%(14/14)。结论电化学发光免疫分析法(ECLIA)检测乙肝两对半定量时若HBsAg处于临界及较低含量的弱反应性标本及感染模式不常见的标本需进行Elecsys HBsAgⅡ确证试验再次检测以防假阴性或假阳性出现。Objective To discuss the clinical significance of HBsAg II confirmatory analysis in detection of HBsAg cutoff and weakly reactive samples with different modes of HBV-M quantitative analysis. Methods We collected serum samples from the out- patients and hospitalized patients form March 2011 to August 2013,and used HBsAg II confirmatory analysis to detect 128 HBsAg cutoff and weakly reactive specimens with diffident models of HBV-M quantitative analysis. Results There were six models of HBV-M quantitative analysis results among the HBsAg cutoff and weakly reactive specimens（Mode I ： HBsAg, HBeAb and HB- cAb,58 samples; Mode II . HBsAg, HBsAb, HBeAb and HBcAb,IIsamples; Mode III .. HBsAg, HBsAb and HBcAb, 8 samples; Mode IV ： HBsAg and HBcAb, 14 samples; Mode V ：HBeAb and HBcAb,23 samples;Mode VI ：HBsAb and HBcAb, 14 samples）. The COI by quantitative analysis of different modes of HBsAg were 1.97,2.15,2.05,2.17,0.89 and 0. 88 , respectively. The com- pliance rates with their Elecsys HBsAg II confirmatory test results were 77.59% （45/58）,81.82%（9/11）,100.00% （8/8）,71. 43% （10/14）, 100.00%（23/23） and 100.00% （14/14） ,respectively. Conclusion If there were HBsAg cutoff and lower levels of weakly reactive samples or samples with unusual infection modes using ECLIA method for detection of HBV-M quantitative analy- sis,the samples should be carried out Elecsys HBsAg II confirmatory test to prevent false negative or false positive results.

关 键 词：肝炎病毒 乙型 肝炎表面抗原 乙型 酶联免疫吸附测定 化学发光测定法 

分 类 号：R512.62[医药卫生—内科学]