耐亚胺培南的阴沟肠杆菌碳青霉烯酶基因检测及同源性分析  

Carbapenemase gene detection and homology analysis of imipenem-resistant Enterobacter cloacae

作　　者：宋景秋[1] 戴蕾[1] 陶月[1] 

机构地区：[1]南京大学医学院附属鼓楼医院检验科,江苏南京210008

出　　处：《国际检验医学杂志》2014年第1期75-76,共2页International Journal of Laboratory Medicine

摘　　要：目的了解碳青霉烯酶基因在耐亚胺培南的阴沟肠杆菌中的分布情况,并进行同源性分析。方法收集耐亚胺培南的阴沟肠杆菌4株,K-B纸片法测定菌株对药物的敏感性;采用改良Hodge试验及EDTA协同实验进行碳青霉烯酶表型检测;PCR法扩增碳青霉烯酶基因,并进行序列分析,ERIC-PCR扩增后电泳进行同源性分析。结果 4株阴沟肠杆菌呈现泛耐药现象;改良Hodge试验阳性4株,金属酶表型试验全部阴性;4株菌均检出KPC-2酶基因,未检出其他碳青霉烯酶基因。同源性分析结果显示4株菌株分为两型。结论引起该院阴沟肠杆菌对碳青霉烯类药物不敏感的首要原因为细菌产KPC-2酶。Objective To study the distribution of carbapenemase gene in imipenem-resistant Enterobacter cloacae,and make ho- mology analysis. Methods Four strains of imipenem-resistant Enterobacter cloacae were collected. The drug sensitivity was meas- ured by the K - B paper method. The modified Hodge test and EDTA collaborative experiments were used to detect carbapenemase phenotype. PCR was used to amplify carbapenemase gene and the sequence was analyzed. ERIC -PCR amplification electrophoresis was clone for homology analysis. Results Four strains occurred pan-resistant Enterobacter cloacae phenomenon, the results of modified Hodge test were all positive and those of metalloenzymes phenotypic test were all negative. The four strains were detected with KPC-2 gene, without the other carbapenemase genes. The four strains were divided into two types by homology analysis. Con- clusion Producing KPC-2 enzyme is the main cause of carbapanem resistance in Enterobacter cloacae in our hospital.

关 键 词：肠杆菌 阴沟 碳青霉烯酶 聚合酶链反应 

分 类 号：R440[医药卫生—诊断学]