4种检测系统检测HBsAg分析灵敏度比较  

Comparison on hepatitis B surface antigen sensitivity determined by four immunoassay systems

作　　者：卢培[1] 黄秋芳[1] 卫晓青 

机构地区：[1]上海市第八人民医院检验科,200235

出　　处：《国际检验医学杂志》2014年第1期80-82,共3页International Journal of Laboratory Medicine

摘　　要：目的评价4种不同的检测系统检测乙型肝炎表面抗原(HBsAg)的分析灵敏度。方法用雅培化学发光免疫分析系统(A)、罗氏电化学发光免疫分析系统(B)、博阳光激化学发光免疫分析系统(C)、科华酶联免疫吸附试验试剂(D)和BioTek酶标仪(ELX-800)等4种检测系统分别测定用健康人血清配制的WHO HBsAg系列浓度标准品和140例临床弱阳性样本,分析比较各检测系统的分析灵敏度,并对结果进行分析。结果 A、B、C、D 4种检测系统cutoff值所对应的WHO参考品的浓度值分别为0.028、0.044、0.187、0.485IU/mL。结论 4种检测系统的分析灵敏度排序为A>B>C>D。Objective To compare the analytical sensitivities of hepatitis B surface antigen（HBsAg） determined by four different immunoassay systems. Methods WHO HBsAg series concentration standard preparations prepared by healthy human serum 140 HBsAg weakly positive samples were measured by four immunoassay systems（a direct chemiluminescence assay-Abbott, system B for electrochemiluminescence assay-Roche,system C light initiated chemiluminescence assay-beyond, D enzyme-linked immunosor- bent assay（ELISA） reader-BioTek ELX-800 and reagent based ELISA-KHB）. The analytical sensitivities of each detection system were analysed and compared. Results The corresponding WHO standard preparations concentration values of the cutoff values de- termined with A.B.C and D detection systems were 0. 028,0. 044,0. 187 and 0. 485 IU/mL respectively. Conclusion The order of the analytical sensitivities determined by four immunoassay systems as follows： A〉B〉C〉D.

关 键 词：肝炎表面抗原 乙型 化学发光测定法 酶联免疫吸附测定 

分 类 号：R446.6[医药卫生—诊断学]