不同产地白芍的指纹图谱对比研究  

Study on fingerprint of different regions paeonia by HPLC

作　　者：张强[1] 杨玉琴[1] 丁秦[1] 杨蝉[1] 

机构地区：[1]贵阳中医学院,贵阳550002

出　　处：《微量元素与健康研究》2014年第1期20-21,23,共3页Studies of Trace Elements and Health

基　　金：贵州省中药管理局基金项目[2007]3号

摘　　要：目的:建立白芍药材的HPLC指纹图谱。方法:高效液相色谱法,色谱柱Diamonsil(钻石)C18(4.6mm×150 mm,5μm);流动相:乙腈(A)和0.05%磷酸水溶液(B);梯度洗脱;检测波长为220 nm;柱温为30℃;流速为1 ml/min,柱温25℃,进样量10μl,洗脱时间为50 min。结果:12批药材共标定了13个共有峰。结论:利用白芍HPLC指纹图谱分析方法较全面地反映白芍药材的内在质量,可用于白芍药材的质量控制。Objective： To determine the fingerprint of Paeonia by HPLC. Methods： The sample was separated on Diamonsil C18, with CH3CN- 0.05% H3PO4 - H2O solution by gradient elution, UV detection wavelength was at 220 nm; Column temperature was at 30℃, the flow fat was 1 ml/min, the elution time for 50 min. Results： 13 common peaks in the HPLC profile were fond in Paeonia in different growing areas. Conclusion：The HPLC fingerprint can be used to control the quality of Paeonia.

关 键 词：白芍 指纹图谱 

分 类 号：R284[医药卫生—中药学]