甘蓝CMS不育系和保持系的电镜观察  

Electron Microscopic Observation on Male Sterile CMSPM and Maintainer Line of Cabbage

作　　者：高营营[1] 王超[1] 张智杰[2] 

机构地区：[1]东北农业大学园艺学院,黑龙江哈尔滨150030 [2]鸡东县农业技术推广中心,黑龙江鸡东230321

出　　处：《北方园艺》2014年第2期15-19,共5页Northern Horticulture

基　　金：国家科技支撑计划资助项目(2012BAD02B01)

摘　　要：为研究甘蓝CMS不育系材料PM的败育机理,以甘蓝不育系PM和保持系PF为试材,用透射电镜观察其小孢子发育的整个过程。结果表明:不育系在花粉母细胞时期形状不规则,严重影响了减数分裂的正常进行;在单核小孢子期,绒毡层细胞不解体且异常膨大,挤压小孢子使其变形,粘连在一起,并出现了严重的质壁分离现象,最后降解的小孢子和绒毡层细胞充斥着整个花粉囊,导致花药败育。Using transmission electron microscopy techniques, the anther and microspore development were studied, in order to obtain differences between CMSPM and its maintainer line of Brassica oleracea L. var. capitata L.. The results showed that pollen mother cells （PMC） of CMSPM was irregular. Thus meiosis of PMC was influenced seriously. During mononuclear stage,the tapetal cells did not degenerated and expand abnormally,pressing the microsporocyte made to be out of shape, and microspore appeared serious plasmolysis phenomenon. Finally degradation of microspore and tapetal cells was full of the whole pollen sac which caused the anther abortion.

关 键 词：甘蓝 胞质不育系 电镜 花药败育 

分 类 号：S635[农业科学—蔬菜学]