梨极矮化突变体与苹果梨GA20ox基因的克隆及植物表达载体的构建  

Cloning and Plant Expression Vector Construction of GA20-oxidase Gene from Pear Dwarfing Mutants and Pingguo Pear

作　　者：王满[1] 金灿[1] 许雪[1] 宗成文[1] 曹后男[1] 

机构地区：[1]延边大学农学院,吉林延吉133000

出　　处：《北方园艺》2014年第2期89-94,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(30960231)

摘　　要：以梨极矮化突变体和苹果梨为试材,应用RT-PCR技术对GA20ox基因进行克隆及序列分析。结果表明:克隆得到梨极矮化突变体和苹果梨GA20-氧化酶基因,依次命名为PuGA20ox和PbGA20ox,其全长均为1 179bp,分别包含1个完整编码392个氨基酸的开放阅读框(ORF);对其进行序列分析,并成功构建了植物表达载体,为进一步研究梨矮化突变体和GA20-氧化酶基因功能奠定了基础。Taking pear dwarf mutamt and ＇Pingguo＇ pear as test material, by RT-PCR technique, cloning and sequencing of GA2Oox gene was studied. The results showed that two GA20-oxidase genes which full-length cDNA consist 1 179 nucleotides and contain complete open reading frame （ORF） which encoding a protein of 392 amino acids residue were isolated from pear dwarfing mutant and ＇Pingguo＇ pear. Moreover named that PuGA20ox and PbGA20ox. Cloning and plant expression vector construction of GA20-oxidase gene from pear dwarfing mutants and ＇Pingguo＇ pear basis for studying the genetic transformation and function of GA20-oxidase gene.

关 键 词：梨极矮化突变体 GA20-氧化酶基因 克隆 序列分析 植物表达载体 

分 类 号：S661.2[农业科学—果树学]