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作 者:冯雪[1] 梁奎景 王茜[2] 张卓文[1] 魏淑珍[2]
机构地区:[1]华中农业大学园艺林学学院,湖北武汉430070 [2]衡水学院生命科学学院,河北衡水053000
出 处:《北方园艺》2014年第2期128-131,共4页Northern Horticulture
基 金:河北省科技支撑计划重点资助项目(11276712D)
摘 要:从培养基种类、温度、光照、pH、微量元素及不同氮源和碳源等方面对合欢枯萎病致病菌的生物学特性影响进行了研究。结果表明:该菌最适生长培养基为PDA培养基和PSA培养基;菌丝生长的温度范围在25-35℃,最适温度为30℃,分生孢子萌发的最适温度为30℃,该菌的致死温度为60℃;菌丝在pH6~12之间均能生长,以pH6时分生孢子萌发率最高;光照对菌丝生长无明显影响,黑暗条件有利于病菌产孢;病菌对蔗糖和可溶性淀粉利用较好,对硝酸钠和硝酸钙利用较好;微量元素中硫酸锰有利于菌丝生长,硫酸亚铁能够较好地促进分生孢子的萌发。The biological characteristics of Fusariurn oxysporum f. sp. of major fungicides of Albizzia julibrissin Durazz were studied from medium,temperature,light,pH,microelement,carbon and nitrate sources. The results showed that F. oxysporum could grow faster in the PDA,PSA,and the basic medium. The optimum temperature of mycelial growth and spore germination were 25-30℃ and the best at 30℃. The lethal temperature of F. oxysporum was 60℃. The optimal pH value of mycelium growth and conidial germination were between 6-12,and 6 was the best. Darkness promoted the growth and spore production of mycelium. Among the tested carbon and nitrogen resources, sucrose, soluble starch, sodium nitrate and calcium sulfate could better facilitate nitrate were useful types. Manganese sulfate was beneficial to spore growth, and ferrous the germination of conidia.
分 类 号:S435[农业科学—农业昆虫与害虫防治]
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