红树植物拟海桑(Sonneratia paracaseolaris)化学成分研究  

Studies on chemical constituents of Sonneratia paracaseolaris

作　　者：程灿玲 宫凯凯 李平林[1] 颜洁[1] 梁振[1] 唐旭利[2] 李国强[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院 [2]中国海洋大学化学化工学院,山东青岛266003

出　　处：《中国海洋药物》2014年第1期53-57,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41076084;20975094)资助

摘　　要：目的研究红树植物拟海桑Sonneratia paracaseolaris的化学成分。方法利用硅胶柱层析、凝胶Sephadex LH-20柱层析、HPLC等手段对化学成分进行了分离纯化；通过理化性质，结合波谱数据分析并与文献对照，鉴定化合物的结构，分别以MTT和SRB法评价化合物的抗肿瘤活性。结果从红树植物拟海桑Sonneratia paracaseolaris的甲醇提取物中，分离鉴定了9个单体化合物：9-氧橙花叔酮（1），3，7，11-三甲基-1，7（E），10-十二碳三烯-3-羟基-9-酮（2），（22E，24R）5cc，8盯过氧化麦角甾23-甲基-6，22-二烯-3伊醇（3），（22E）-5α，8α-过氧化麦角甾-23甲基-6，9，22-三烯-3β-醇（4），（22E）5α，8α-过氧化麦角甾-6，22-二烯-3β-醇（5），4’-甲氧基偏柏脂素（6），偏柏脂素（7），3’-羟基-4’-甲氧基-4’-脱羟基偏柏脂素（8），1，3二对羟基苯基-4-戊烯-1-酮（9）。化合物6～9对A549、HL-60、P388及K562肿瘤细胞株显示出不同程度的抑制作用，其中化合物8对K562、HL60细胞具有较强的细胞毒活性，IC50值分别为1．99、3．13μmol／L。结论化合物1～9均为首次从拟海桑Sonneratia paracaseolaris中分离得到；首次测定了化合物6～9对A549、HL-60、P388及K562肿瘤细胞株的细胞毒活性，其中化合物8对HL-60、K562细胞显示出较好的抑制活性。Objective To study the chemical constituents of Sonneratia paracaseolaris. Methods The isolation and purification of the compounds were performed by silica gel, Sephadex LH-20 and HPLC methods, their structures were determined by comparison their physieoehemical characters and spectral data with literatures, and their antitumor activities were evaluated by MTT and SRB methods respec- tively. Results From the methanol extracts of Sonneratia paracaseolaris, nine compounds were isolated and identified as 9-oxonerolidol （1）, 3, 7, 11-trimethyldodeca-1, 7（E）, 10-trien-3-ol-9-one （2）, （22E,24R）-5α, 8α-epidioxy-23-methylergosta-6, 22-dien-3β-ol （3）, （22E, 24R）-5α, 8α-epidioxy-23-methylergosta-6, 9, 22-trien-3β-ol （4）, （22E）-5α, 8α-epidioxy-6, 22-dien-3β-ol （5）, 4＇-O-methyl-nyasol （6）, nyasol （7）, 3 -hydroxy-4 -methoxy-4-dehydroxynyasol （8）, 1, 3-di-p-hydroxyphenyl-4-penten-1 one （9）. Compounds 6-9 showed inhibitory activities against tumor cell lines including A549, HL-60, P388 and K562, and compound 8 had more potential antitumor activities against K562 and HL-60 cells,with ICs0 values of 1.99, 3. 13 mol/L, respectively. Conclusion Compounds 1 -9 were isolated from this Sonneratia paracaseolaris for the first time; Compounds 6-9 were evaluated their cytoxic activi ties against A549, HL-60, P388 and K562 cell lines for the first time, and compound 8 exhibited more potential inhibitory activities against HL-60 and K562 cell lines.

关 键 词：拟海桑 化学成分 结构鉴定 

分 类 号：R931[医药卫生—生药学]