黄瓜异戊烯基转移酶基因(CsIPT2)的克隆及特征分析  

Isolation and characterization of isopentenyl transferase gene in Cucumis sativus(CsIPT2)

作　　者：张停林[1] 崔利[1] 李季[1] 郭勤卫[1] 娄群峰[1] 徐建[1] 陈劲枫[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏南京210095

出　　处：《南京农业大学学报》2014年第1期21-26,共6页Journal of Nanjing Agricultural University

基　　金：国家973计划项目(2012CB113904;2009CB119001-01);高等学校博士学科点专项科研基金项目(20120097120037);江苏省农业科技支撑计划重大项目(BE2012323)

摘　　要：为了分析黄瓜异戊烯基转移酶基因(CsIPT2)在果实发育不同模式中的转录变化以及对不同激素的转录应答情况,对CsIPT2基因进行了克隆和序列分析,再利用荧光定量PCR技术分析该基因在黄瓜单性结实自交系EC1和非单性结实自交系8419s-1果实发育过程中的表达情况,并分析了CsIPT2对不同激素的应答反应。结果表明:CsIPT2基因全长843 bp,共编码280个氨基酸,蛋白空间结构模型与蛇麻IPT蛋白极其相似。该基因不仅在授粉后的黄瓜果实发育中表达量上调,而且在黄瓜天然单性结实前期和人工诱导的单性结实后期表达量均有增加,推测该基因在促进黄瓜果实发育过程中发挥着重要作用,同时也可能参与了黄瓜单性结实的调控过程。GA、ET、6-BA、ABA和NAA 5种激素都能够诱导该基因的上调表达。其中GA处理后3 h基因表达量最高,ET、6-BA、ABA和NAA诱导的表达峰值出现在处理后6 h。随着NAA处理浓度的增加基因的表达量上调幅度增大。To analyze the role of isopentenyl transferase gene in Cucumis sativus（CslPT2） during fruit development of cucumber and response after different hormones treatment, based on cloning and sequence analysis of CslPT2 gene, parthenocarpic line EC1 and non-parthenocarpic line 8419s-! were used for real-time RT-PCR analysis. The results showed that the length of CslPT2 gene was 843 bp, encoding 280 amino acids. The protein structure model was extremely similar to IPT protein of hop （ Humulus lupulus L. ）. The expression of gene was higher during the fruit development of cucumber after pollination. At the same time the expression of CslPT2 was higher at the early stage of natural parthenocarpic fruit and the late stage of artificially induced parthenocarpic fruit. Therefore,it was speculated that CslPT2 might play an important role in promoting parthenocarpy of cucumber. Moreover, the five hormones （GA, ET,6-BA, ABA and NAA）were able to up-regulate the expression of the gene, The highest expression of ET,6-BA, ABA and NAA appeared 6 h after treatment, while only GA did 3 h after treatment. The higher the concentration the higher the expression during the NAA treatment.

关 键 词：黄瓜 黄瓜异戊烯基转移酶基因(CsIPT2) 克隆 表达分析 

分 类 号：S642.2[农业科学—蔬菜学]