星点设计-效应面法优化O-羧甲基壳聚糖复凝海绵对凝血酶的固定化作用  

Optimization of Immobilized Effect of the O-Carboxymethyl Chitosan Multi-Hemostatic Sponge on Thrombin by the Central Composite Design-Response Surface Methodology

作　　者：吴迪[1,2] 齐同珍 刘辉[1] 浦金辉[1] 张婧[1,2] 邹萍[1,2] 

机构地区：[1]广州军区武汉总医院药剂科,武汉430070 [2]湖北中医药大学药学院,武汉430065 [3]荆州市第三人民医院药剂科,湖北荆州434001

出　　处：《中国药学杂志》2014年第2期138-142,共5页Chinese Pharmaceutical Journal

基　　金：全军医学科研"十二五"计划课题(CWS11C274)

摘　　要：目的考察O-羧甲基壳聚糖复凝海绵对凝血酶的固定化作用。方法以水溶性O-羧甲基壳聚糖为载体、戊二醛为交联剂,加入凝血酶作为复凝血剂,采用星点设计-效应面法优化O-羧甲基壳聚糖复凝海绵处方,通过测定固定化凝血酶的活性回收率,考察凝血酶的固定化条件。结果 O-羧甲基壳聚糖复凝海绵固定凝血酶的最优处方为:O-CMC 2.49%;凝血酶49.87 U·mL-1;戊二醛0.49%。结论 O-羧甲基壳聚糖复凝海绵对凝血酶有良好的固定化作用。OBJECTIVE To investigate the effects of O-carboxymethyl chitosan （O-CMC） muhi-hemostatic sponge on the immo- bilization of thrombin. METHODS The formulation of 0-CMC muhi-hemostatic sponge was optimized by using central composite de- sign-response surface methodology （CCD-RSM）, in which the O-CMC, glutaraldehyde and thrombin were used as enzyme immobiliza- tion carrier, crosslink agent and coagulant, respectively. The crosslinked conditions of thrombin were studied by determining the enzy- matic activity yield. RESULTS The optimized concentrations of O-CMC, thrombin and glutaraldehyde were 2.49%, 49. 87 U· mL-1 , 0. 49% , respectively. CONCLUSION O-CMC multi-hemostatic sponge has the positive immobilization effect on thrombin.

关 键 词：O-羧甲基壳聚糖 凝血酶 固定化 活性回收率 星点设计-效应面法 

分 类 号：R944[医药卫生—药剂学]