丙酸质量浓度对丙酸富集培养物降解特性的影响  

Effect of propionate concentration on degradation characteristics of a propionate enriched culture

作　　者：班巧英[1,2] 李建政[1] 张立国[2] 张玉鹏[1] Ajay Kumar Jha 艾斌凌[1] 

机构地区：[1]哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090 [2]山西大学环境与资源学院,太原030006

出　　处：《哈尔滨工业大学学报》2013年第12期43-47,共5页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金资助项目(51178136);城市水资源与水环境国家重点实验室自主课题(2010DX06)

摘　　要：为阐明丙酸质量浓度对厌氧生物处理系统中丙酸降解特性的影响，研究了不同丙酸质量浓度条件下丙酸富集培养物的降解特性．以丙酸为唯一碳源，通过15代的传代培养富集到一个中温互营丙酸氧化菌群（包括丙酸氧化菌和产甲烷菌）．聚合酶链式反应一变性梯度凝胶电泳（PCR—DGGE）指纹分析结果表明，该富集培养物中的丙酸氧化菌为Syntrophobacter，而嗜氢产甲烷菌和嗜乙酸产甲烷菌分别为Methanobacterium和Methanosaeta．在污泥接种量为0．81g／L条件下，当丙酸质量浓度为1000—2000mg／L时，丙酸能够被该富集培养物快速降解；而当丙酸质量浓度为2500—3000mg／L时，丙酸降解和乙酸的转化均受到一定程度的抑制，但随着培养时间的延长，该抑制作用逐步解除．较高的丙酸质量浓度（≥2500mg／L）能够对丙酸氧化菌产生可逆性抑制作用．To clarify the effect of propionate concentration on propionate degradation, propionate degradation feature at different propionate concentration conditions was investigated. A mesophilic syntrophic propionate- oxidizing bacterial community （containing propionate-oxidizing bacteria and methanogens） was firstly obtained by 15 times subculture using propionate as sole carbon source. Polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） revealed that the main propionate-oxidizing bacteria belonged to genus Syntrophobacter while hydrogenotrophic methanogens and aceticlastic methanogens were related to Methanobacterium and Methanosaeta, respectively. Then the batch test was performed for evaluating the effect of propionate concentration on syntrophic propionate degradation. The results showed that the propionate was rapidly degraded at propionate concentration of 1 000-2 000 mg/L under the inoculated sludge of 0. 81 g/L condition. However, the propionate degradation was inhabited when the propionate concentration was 2 500- 3 000 mg/L.The inhibition was gradually relieved with extension of time. It implied that high concentration propionate （ ≥2 500 mg/L） would inhibit the metabolic activity of propionate-oxidizing bacteria and this inhibition was reversible.

关 键 词：丙酸 富集培养物 丙酸氧化菌 产甲烷菌 降解特性 

分 类 号：X703[环境科学与工程—环境工程]