高温胁迫下百脉根生理生化响应及耐热性评价  

Physiological response and heat tolerance evaluation of Lotus corniculatus under high temperature stress

作　　者：赵丽丽[1] 王普昶 陈超[1] 马林[1] 

机构地区：[1]贵州大学动物科学学院草业科学系,贵州贵阳550025 [2]贵州省草业研究所,贵州贵阳550025

出　　处：《草业科学》2013年第12期2018-2023,共6页Pratacultural Science

基　　金：贵州省科学技术基金项目[黔科合J字(2010)2248];贵州省科技厅项目[黔科合NY(2010)3049号];贵州大学引进人才科研项目[贵大人基合字(2010)036号];贵州省科教青年英才培育工程项目[黔省专合字(2012)142号]

摘　　要：以8个百脉根(Lotus corniculatus)种质资源为试验材料,在不同高温胁迫下,测定分析了各种质叶片的相对电导率值、丙二醛(MDA)含量、游离脯氨酸(Pro)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性和过氧化氢酶(CAT)活性6项指标,采用主成分分析与隶属函数相结合的方法对种质间耐热性进行综合评价。结果表明,随高温胁迫增强,百脉根各种质的相对电导率、MDA和Pro含量增加,CAT和POD酶活性(除B01种质外)呈升高趋势,SOD酶活性呈先升高后降低趋势。通过主成分分析可从测定的6个单项指标中抽取出两个相互独立的综合指标,累积贡献率达85.41%。综合3个温度梯度下8个百脉根种质资源的综合评价(D)值,可以推测出各种质耐热性顺序为B08>B04>B05>B18>B03>B12>B02>B01。In order to study physiological response and evaluate heat tolerance of Lotus corniculatus under high temperature stress, leaf relative electrolyte leakage, contents of malondialdehyde（MDA）, proline （Pro） and activities of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） were analyzed using 8 germplasms of L. corniculatus under different temperature treatments （35 ℃ ,45 ℃, and with 22 ℃ as control）. The heat tolerance of 8 germplasms was comprehensively evaluated by principal compo- nents analysis and fuzzy subordinate function based on the tested 6 indices. The leaf relative electrolyte leakage, MDA content, proline content, activities of catalase （CAT） and peroxidase （POD） increased with the temperature increasing except germplasm B01 which did not have higher POD activity at higher temper- ature. The activity of superoxide dismutase （SOD） was higher at moderate temperature （35 ℃） compared with the other two temperature treatments. Principal component analysis was applied to analyze the total effects of these six tested indices which showed that the first and second compont of PCA contributed to 85.41%. Based on the comprehensive evaluation value （D）, the heat tolerance of these eight L. cornicula- tus germplasms was estimated as B08〉B04〉B05〉B18〉B03〉B12〉B02〉B01.

关 键 词：百脉根 生理生化指标 主成分分析 隶属函数分析 耐热性 

分 类 号：S541.9[农业科学—作物学]