绒山羊细管冻精的解冻方法  

Study on thawing methods of straw frozen semen in cashmere goats

作　　者：陈倩[1] 丛玉艳[1] 刘兴伟 宋先忱 

机构地区：[1]沈阳农业大学畜牧兽医学院,辽宁沈阳110866 [2]辽宁省畜牧科学院,辽宁辽阳110000

出　　处：《草业科学》2013年第12期2066-2070,共5页Pratacultural Science

基　　金：国家科技支撑计划"常年长绒型辽宁绒山羊新品系选育扩繁及产业化示范"(2009BADA5B02)

摘　　要：将6只2周岁的辽宁绒山羊种公羊精液混合后制成0.25mL细管冻精,分别采用37℃12s、39℃8s、70℃4s3种方法进行解冻,通过对精子活力、精子存活时间、顶体完整率、畸形率和精清酶进行分析,并结合升温曲线,研究解冻方法对绒山羊细管冻精品质的影响。结果发现,70℃解冻4s的精子活力显著高于37℃12s(P<0.05),与39℃8s相比差异虽不显著(P>0.05),但亦有增高的趋势;不同解冻方法对解冻后精子存活时间的影响差异不显著(P>0.05);70℃解冻4s的精子顶体完整率显著高于其余两种解冻方法(P<0.05),且精子畸形率显著低于其余两种解冻方法(P<0.05);70℃解冻4s的精清中GOT活性显著低于其余两种解冻方法(P<0.05),且LDH活性和HYD含量均显著低于37℃12s(P<0.05);70℃下解冻4s的精子经过危险温区速率最快。因此,70℃4s解冻方法能有效缓解解冻对精子的损伤,对保持精子的正常形态和活力较为有利,是绒山羊细管冻精适宜的解冻方法。The semen of six 2-year-old liaoning cashmere bucks were collected and mixed to gain some 0.25mL straw frozen semen. Three thawing methods （37 ℃ 12 s, 39 ℃ 8 s, 70 ℃ 4 s） were respectively ap- plied to thaw the frozen semen as mentioned above. Sperm vitality, sperm survival time, acrosome intact rate, deformity rate, enzymes in seminal plasma of post-thawed semen and the heating curve were analyzed to study the effects of the thawing methods on sperm quality of straw frozen semen in cashmere goats. The results show that, sperm vitality of the frozen semen after thawing for 4 s under 70 ℃ was significantly higher than that of 12 s under 37 ℃ （P〈0.05）, and it was higher than that of 39 ℃ 8 s which was not sig- nificantly （P〉0.05） ; There were no significant difference between the survival time of the thawed sperm among three thawing methods （P〉0.05） ; the intact rate of sperm acrosome thawed with 70 ℃ for 4 s was significantly higher than that of the other two thawing methods （P〈0.05）, while the sperm deformity rate thawed with 70 ℃ for 4 s was significantly lower than the other two thawing methods of （P%0.05） the GOT activity thawed with 70 ℃ 4 s was significantly lower than that of the other two thawing methods （P%0.05）, and the LDH activity and HYD content thawed with 70 ℃ 4 s was significantly lower than that of 37 ℃ 12 s （P〈0.05） ; the semen thawed with 70 ℃ 4 s went through the danger zones at the high- est velocity. Therefore, the thawing method of at 70 ℃ for 4 s is suitable for thawing straw frozen semen of cashmere goats.

关 键 词：解冻方法 细管冻精 精子品质 酶 绒山羊 

分 类 号：S827[农业科学—畜牧学]