不同产地苦丁茶多糖的提取  

Extraction of polysaccharide fromIlex kuding cha C.J.Tseng from different habitats

作　　者：黄敏桃 吴尤娇[1,2] 黄云峰[3] 赖茂祥[3] 黄庶识[1] 刘华钢[4] 

机构地区：[1]广西科学院生物物理实验室,广西南宁530007 [2]广西中医药大学药学院,广西南宁530001 [3]广西中医药研究院,广西南宁530022 [4]广西医科大学,广西南宁530021

出　　处：《食品与机械》2013年第6期166-170,共5页Food and Machinery

基　　金：国家科技支撑计划项目(编号:2011BAI01B04);广西科学研究与技术开发计划(编号:0992003A-20);广西科学研究与技术开发计划项目(编号:桂科重1298001-2-3)

摘　　要：研究苦丁茶中多糖的提取工艺条件，采用响应面法优化工艺参数，并利用苯酚-硫酸比色法测定不同产地苦丁茶中的多糖含量。结果表明：不同产地苦丁茶多糖含量具有显著差异，13批不同产地苦丁茶多糖含量为39．991～88．437mg／g，以广西大新县苦丁茶多糖含量最高。同一品种苦丁茶中的多糖含量7月份的比12月份的高；人工栽培的苦丁茶中多糖含量明显高于野生品种。Using the response surface methodology to define the opti- mum technology and Phenylhydrate-sulfuric acid method to deter- mine the content of the total polysaccharides of Ilex kudingcha C. J. Tseng grown in different habitats. The result showed there was a significant difference in polysaccharide content from Ile3： kudingcha C. J. Tseng from different localities, and the total polysaccharide content of Ilex kudingcha C. J. Tseng in 13 different localities was： 39. 991 ~ 88. 437 rag/g, then the highest of this was Daxin from Guangxi. Content of total polysaceharides from the same habi- tat in July was higher than in December and from artificial cultivation was significantly higher than in wild species.

关 键 词：苦丁茶 多糖 产地 野生 苯酚-硫酸比色法 

分 类 号：R284[医药卫生—中药学]