斜生栅藻对草甘膦异丙胺盐的毒性响应  

Acute and Chronic Response of Scenedesmus obliquus to Glyphosate-isopropylammonium

作　　者：邱伟建[1] 陈敏东[1] 葛顺[2] 宋玉芝[1] 龚旋[1] 周军英 单正军 

机构地区：[1]南京信息工程大学环境科学与工程学院,江苏省大气环境监测与污染控制高技术重点实验室,江苏南京210044 [2]江苏省环境监测中心,江苏南京210036 [3]环保部南京环境科学科学研究所,江苏南京210042

出　　处：《环境科学与技术》2013年第12期24-28,共5页Environmental Science & Technology

基　　金：环保公益性行业科研专项(201009033)

摘　　要：为了解有机磷农药毒死蜱污染对本土水生敏感性物种斜生栅藻的毒性效应,在实验室条件下采用静态毒性实验。研究了草甘膦异丙胺盐对斜生栅藻96 h急性毒性效应,并在急性试验基础上分析了不同浓度草甘膦异丙胺盐存在14 d后斜生栅藻叶绿素含量、可溶性蛋白以及丙二醛含量。结果表明:草甘膦异丙胺盐的96 h EC50为619.12 mg/L,属低毒农药;草甘膦异丙胺盐对斜生栅藻叶绿素a抑制作用显著,故叶绿素a可作为毒性评价敏感性指标;斜生栅藻在前期短时间低浓度胁迫作用下能促进可溶蛋白、MDA含量的上升,但在后期长时间高浓度的胁迫作用下,对斜生栅藻产生实质性的损伤至死亡,以胁迫作用后期可溶蛋白含量和MDA含量的下降体现出来。In order to understand the effect of pollution by chlorpyrifos of organophosphorus pesticide on the Scenedesmus obliquus, 96 h acute response of Scenedesmus obliquus to chlorpyrifos was investigated on the indoor condition using static toxicity test. Based on the acute environment and chronic environment, analysis was done to show the content of Chlorophyll a, solute protein and MDA in Scenedesmus obliquus when they were exposed to different concentrations of chlorpyrifos after 14 d. Results showed that 96 h ECso of glyphosate-isopropylammonium was 619.12 mg/L which belongs to lowly toxic pesticide. Since glyphosate-isopropylammonium＇ s impact on the Chlorophyll a of Scenedesmus obliquus is obvious, it could be used as a sensitive indicator of toxicity evaluation. Low concentration of glyphosate-isopropylammonium could promote the content of solute protein and MDA in the early experiments, while under long time and high concentration duress in the later experiments, Scenedesmus obliquus have produced substantial injury to death that shows by the content of solute protein and MDA decrease.

关 键 词：草甘膦异丙胺盐 斜生栅藻 EC 叶绿素A 可溶蛋白 MDA 

分 类 号：X503.2[环境科学与工程—环境工程]