单核细胞趋化蛋白-1基因-2518A／G多态性与Tourette综合征的关联性  

Association between monocyte chemoattractant protein 1 gene .2518A/G polymorphisms and Tourette syn- drome

作　　者：张颖[1] 匡桂芳 王桂菊[4] 韩振靓[5] 车峰远[6] 马小旭[7] 刘世国 衣明纪[1] 

机构地区：[1]青岛大学医学院附属医院儿童保健科,266003 [2]山东省代谢重点实验室 [3]青岛市妇女儿童医院心理科 [4]日照市人民医院儿童保健科 [5]青岛大学医学院附属医院东区儿科 [6]临沂市人民医院儿科 [7]东营市胜利油田中心医院儿科

出　　处：《中华实用儿科临床杂志》2013年第24期1860-1863,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（30971586,81371499）

摘　　要：目的探讨山东地区汉族人群单核细胞趋化蛋白-1基因（MCP-1基因）-2518A／G位点多态性与Tourette综合征（TS）发病之间的关系。方法应用聚合酶链反应．限制性片段长度多态性对山东地区汉族人群193例TS患儿（TS组）和252例健康对照者（健康对照组）进行MCP-1 基因-2518A／G位点多态性检测，采用病例对照研究的方法，分析MCP-1基因-2518A／G多态性与TS发病间的关系。结果MCP-1基因-2518A／G位点GG基因型、AA基因型和AG基因型在TS组中频率分布分别为29．5％、19．7％和50．8％，在健康对照组中频率分布分别为33．7％、21．0％和45．3％，基因分布均符合Hardy—Weinberg平衡检验（P〉0．05）；GG、AA和AG3种基因型在2组间分布比较，差异均无统计学意义（P均〉0．05）。G和A等位基因在Ts组中频率分布分别为54．9％和45．1％；健康对照组中为56．3％和43．7％，2组间分布差异均无统计学意义（P均〉0．05）。结论MCP-1基因-2518A／G多态性与TS发病无显著关联，但仍需扩大样本量、分严重程度在不同种族中进行验证。Objective To study the relationship between monocyte chemoattractant protein-1 （MCP-1）gene -2518A/G polymorphisms and Tourette syndrome（TS） in Han population of Shandong Province. Methods One hun- dred and ninety-three TS patients（ TS group） and 252 healthy individuals （healthy control group） were collected from Han population of Shandong Province. Polymerase chain reaction and restriction enzyme digestion were used for determi- ning the genotype of MCP-1-2518A/G polymorphisms. The data were analyzed by using the case-control study to re- search the relationship between MCP-1-2518A/G polymorphisms and TS. Results The frequency distributions of GG, AA and AG genotype of MCP-1-2518A/G in TS patients were 29.5%, 19.7% and 50.8% , and in healthy controls they were 33.7% ,21.0% and 45.3% ,respectively. All the data were in accordance with Hardy-Weinberg equilibrium （ P 〉 0.05 ）. The genotype distributions of GG, AA and AG genotypes were compared separately between the 2 groups, and there was no statistically significant difference （ all P 〉 0.05 ）. The frequency distributions of G allele and A allele in TS patients were 54.9% and 45.1% , and in healthy controls they were 56.3% and 43.7%. No significant diffe- rence was found between the 2 groups （ all P 〉 0.05 ）. Conclusions There is no significant association between MCP-1-2518A/G polymorphisms and TS. However,further study is still needed by expanding the sample size, stratifying patients according to the disease severity and collecting patients in different races.

关 键 词：TOURETTE综合征 单核细胞趋化蛋白-1 病例对照研究 

分 类 号：R725.9[医药卫生—儿科]