CMV-PP65抗原检测在预防肾移植术后巨细胞病毒感染的临床价值  

The Clinical Value of CMV-PP65 Antigen Detection in the Prevention of Cytomegalovirus Infection After Renal Transplantation

作　　者：谭湘芳[1] 罗志刚[2] 王毅[2] 李建军[2] 钱坤[2] 李跃进[1] 

机构地区：[1]南华大学附属第二医院检验科,湖南衡阳421001 [2]南华大学第二附属医院肾移植科

出　　处：《中南医学科学杂志》2013年第6期570-571,626,共3页Medical Science Journal of Central South China

基　　金：衡阳市科委资助课题(2008KS022)

摘　　要：目的 探讨巨细胞病毒PP65抗原（CMV-PP65）检测在预防肾移植术后巨细胞病毒（CMV）感染的临床价值.方法 采用间接免疫荧光法和酶联免疫捕获法检测52例肾移植受者术后CMV-PP65抗原和CMV-IgM抗体.结果 52例肾移植受者中CMV-PP65抗原检测阳性25例,CMV-IgM抗体检测阳性17例,随访3~6个月,共15例发生CMV病,CMV-PP65抗原检测均阳性,CMV-PP65抗原检测的敏感度及阴性预测值均高于CMV-IgM抗体检测;25例患者CMV-PP65抗原检测术后首次出现阳性的平均时间为4.2±3.3周,17例患者CMV-IgM抗体检测术后首次出现阳性的平均时间为8.7±5.8周,两者比较有显著差异性（P〈0.05）.结论 CMV-PP65抗原检测可早期排除和预防肾移植术后CMV感染,值得临床推广应用.Objective To investigate the clinical value of CMV-PP65 antigen detection in the prevention of cytomeg- alovirus infection after renal transplantation. Methods The CMV-PP65 antigen and CMV-IgM antibody of 52 cases with renal transplantation recipients were detected with the method of indirect immunofluorescence and enzyme-linked immune capture. Results Among the 52 cases of kidney transplant ,25 cases were tested positive with CMV-PP65 antigen, 17 cases were tested positive with CMV-IgM antibody. All patients were followed up for 3 -6 months, CMV occurred in 15 cases, and their CMV-PP65 antigen were detected positive, the detection sensitivity and negative predictive value of CMV-PP65 an- tigen detection were higher than that of CMV-IgM antibody detection. The average length for the first postsurgical positive detection of CMV-PP65 antigen with 25 cases was 4.2 ± 3.3 weeks, while that of the CMV-IgM antibody with 17 cases was 8.7 ± 5.8 weeks, with a significant difference between them （ P 〈 0.05 ）. Conclusion CMV-PP65 antigen detection could be employed for the early elimination and prevention of CMV infection after renal transplantation. It was worthy of clinical popularization and application.

关 键 词：肾移植 巨细胞病毒PP65 巨细胞病毒IgM抗体 

分 类 号：R446.6[医药卫生—诊断学]