微乳液毛细管电动色谱法测定雷公藤炮制品中雷公藤甲素的含量  

Determination of Triptolide in Processed Products of Tripterygium Wilfordii by Microemulsion Electrokinetic Chromatography

作　　者：丛日琳 林诗瑶 褚克丹[1] 徐伟[1] 余丽双[1] 

机构地区：[1]福建中医药大学药学院,福建福州350108

出　　处：《聊城大学学报（自然科学版）》2013年第4期58-61,共4页Journal of Liaocheng University:Natural Science Edition

基　　金：国家自然科学基金(81202913);福建省科技重大专项子专题(2009YZ0001-1-1);福建省科技厅重点项目(2013Y0059);福建省科技厅项目(2010J05020;2012D009)资助

摘　　要：利用微乳液毛细管电动色谱法测定不同雷公藤炮制品中雷公藤甲素的含量.在含1%(w/v)十二烷基硫酸钠(SDS),3%(v/v)正丁醇,1%(v/v)乙酸乙酯和96%(v/v)5mmol/L硼砂-10mmol/L磷酸二氢钠溶液(pH 8.5)的微乳体系下,雷公藤炮制品中的雷公藤甲素在10min内得到良好的分离与检测.不同雷公藤炮制品中雷公藤甲素含量的测定结果:其中生品中的含量最高,为2.008 5g/g,其余炮制品雷公藤甲素的含量分别为清炒品1.716 6g/g,酒炙品1.541 2g/g,水煮品1.758 4g/g.In the study, triptolide was determined in different processed products of Tripterygium wilfordii by microemulsion electrokinetic chromatography. The microemulsion was composed of 1% （w/v） sodium dodecyl sulfate （SDS）, 3% （v/v） n-butanol, 1% （v/v） ethyl acetate and 96% （v/v） 5 mmol/L borax-10 mmol/L phosphate-sodium at pH 8.5. In the microemulsion system, triptolide in tripterygium processed products achieved a good separation and determination within 10 min. This method was used to determinate content of triptolide in different tripterygium processed products, the result showed, crude slices was higher than others. The content of triptolide in crude slices was 2. 008 5 g/g, clear fried slices 1. 716 6 g/g, wine fried slices 1. 541 2 g/g, water boiled slices 1. 758 4 g/g.

关 键 词：微乳液毛细管电动色谱 雷公藤甲素 炮制品 

分 类 号：O657.8[理学—分析化学]