促红细胞生成素衍生肽对顺铂所致大鼠急性肾损伤的剂量反应关系的探讨  

Dose Response Relationship between Helix B Surface Peptide and Cisplatin-induced Acute Kidney Injury

作　　者：柴璐[1] 王锋[1] 范亚平[1] 杨斌[1] 徐玉音[1] 周建萍[1] 

机构地区：[1]南通大学附属医院肾内科,江苏省南通市226001

出　　处：《中国全科医学》2013年第36期4288-4292,共5页Chinese General Practice

基　　金：国家自然科学基金资助项目(81170689);2011_S社会事业科技创新与示范计划(HS2011029)

摘　　要：目的研究促红细胞生成素衍生肽(HBSP)对顺铂所致大鼠急性肾损伤(AKI)的肾脏保护作用的剂量反应关系,进一步探讨HBSP在AKI的使用方法。方法清洁级健康SD大鼠随机分为5组,分别为:对照组(5只)、顺铂组(20只)、HBSP(8 nmol/kg)组(20只)、HBSP(16 nmol/kg)组(20只)、HBSP(32 nmol/kg)组(20只)。应用全自动生化分析仪检测大鼠血尿素氮、肌酐、血红蛋白,苏木素-伊红(HE)染色观察肾组织的病理变化及TUNEL法检测肾小管上皮细胞凋亡。结果各组大鼠不同时间(实验前及实验的第1、3、5、7天)血尿素氮及肌酐水平比较,差异均有统计学意义(尿素氮:F组间=441.456,F时间=10.643,P<0.001;肌酐:F组间=417.933,F时间=9.412,P<0.000 1);各组大鼠不同时间血红蛋白水平比较,差异无统计学意义(F组间=566 645.213,F时间=1.581,P=0.200)。各组大鼠不同时间肾组织TUNEL染色百分比比较,差异有统计学意义(F组间=226.100,F时间=9.553,P<0.000 1)。结论 HBSP对顺铂所致AKI的肾脏保护作用可能与使用剂量有关。Objective To investigate dose response relationship between helix B surface peptide （HBSP, a kind of erythropoietin -derived peptide） and cisplatin -induced acute kidney injury （AKI） in rats, so as to the usage of HBSP in treat- ment of AKI could be further discussed. Methods Healthy Sprague - Dawly （SD） rats of clean grade were randomly divided into5 groups： control group （5）, cisplatin group （20）, HBSP group （8 nmol/kg） （20）, HBSP group （16 nmol/kg） （：20）, HBSP group （32 nmol/kg） （20） . Serum creatinine, urea nitrogen and hemoglobin were detected by automatic bio- chemistry analyzer, the renal tissues were stained by HE to reflect the degree of renal pathology, apoptosis of tubular epithelium was detected by TUNEL assay. Results At various time points （ before experiment and at 1, 3, 5, 7 day after experiment）, there were significant differences in serum creatinine and urea nitrogen levels between groups （BUN： Fgroup = 441. 456, Ftime = 10. 643, P 〈0. 001 ; Scr： Fgroup =417. 933, Ftime = 9. 412, P 〈 0. 000 1 ）, there was no significant difference in hemoglobin levels between groups （Fgroup = 566 645. 213, Ftime = 1.581, P = 0. 200）, there was significant difference in percentages of kid- ney tissue TUNEL staining levels （ Fgroup = 226. 100, Ftime = 9. 553, P 〈 0. 000 1 ） . Conclusion The renal protective effects of HBSP on cisplatin - induced acute kidney injury may be related with HBSP doses.

关 键 词：促红细胞生成素衍生肽 顺铂 急性肾损伤 血尿素氮 肌酐 

分 类 号：R692.5[医药卫生—泌尿科学]