水霉菌蛋白制备方法的比较与分析  

Comparison and Analysis of Preparation Methods of Saprolegnia Protein

作　　者：宋鹏鹏[1] 胡鲲[1] 杨先乐[1] 

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306

出　　处：《湖北农业科学》2013年第21期5342-5344,共3页Hubei Agricultural Sciences

基　　金：国家高技术研究发展计划项目(2011AA10A216);公益性农业行业专项项目(201203085);上海市科技兴农重点攻关项目(沪农科攻字[2011]第4-8号);国家科技基础条件平台项目资助

摘　　要：比较了3种方法对8种水霉菌(Saprolegnia)破壁处理制备蛋白的效果,并进行聚丙烯酰胺凝胶电泳分析。结果表明,加石英砂和液氮混合研磨处理制备的蛋白效果最好,条带清晰,该方法操作简便、快捷、较为常用,可有效地区分水霉菌株分子遗传学背景的差异,适用于水霉菌分子流行病学研究。The effect of three wall-breaking methods of 8 kinds of Saprolegnia preparation was compared and analyzed by polyacrylamide gel electrophoresis. The results showed that the quartz sand and liquid nitrogen mixed grinding processing preparation of protein was best, the stripe was clear, the method was simple, quick, and relatively common, which could effectively distinguish between strains of molecular genetics background difference and was suitable for Saprolegnia molecular epidemiology research.

关 键 词：水霉菌(Saprolegnia) 聚丙烯凝胶电泳 处理方法 

分 类 号：S941[农业科学—水产养殖]