一株产油酵母菌的紫外诱变选育  

Breeding a Strain of Oleaginous Yeast via UV Induced Mutation

作　　者：马勇[1] 图雅[1] 刘晓光[1] 

机构地区：[1]包头师范学院生物科学与技术学院,内蒙古包头014030

出　　处：《湖北农业科学》2013年第22期5463-5465,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31160254);校内青年科学研究基金项目(BSYKJ2011-17)

摘　　要：以产油酵母菌Y1为试验菌株,通过确定出发菌株Y1的最佳紫外诱变时间,诱变后菌株的初筛和复筛,得到了一株突变菌株Y9。测得Y9的油脂产量为5.25 g/L,油脂含量为23.85%。与菌株Y1油脂产量2.11 g/L、含油量9.6%相比,Y9的产油量有明显提高,因此拟选取油脂产量较高的Y9作为后续摇瓶发酵产脂研究的实验菌株。Oleaginous yeast Y1 was used as material. With the best time of UV mutagenesis by the starting strain Y1, a mutant strain Y9 was obtained by strain screening and rescreening after mutagenesis. The oils production of Y9 was 5.25 g / L with oil content of 23.85%. Comparing to Y1 with the oil production of 2.11 g / L and oil content of 9.6%, the oil production of Y9 increased significantly. Oleaginous Yeast Y9 could be used as the laboratory strain for further studying flask fermenta- tion to produce oils.

关 键 词：产油酵母 紫外诱变 诱变选育 油脂产量 

分 类 号：Q93-331[生物学—微生物学]