肉质相关基因TCAP启动子与转录因子MyoD结合的ChIP分析  

Detection of MyoD Transcription Factor Binding with TCAP Gene Promoter by Chromatin Immunoprecipitation

作　　者：吴俊静[1] 梅书棋[1] 彭先文[1] 乔木[1] 武华玉[1] 刘贵生[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所/动物胚胎与分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2013年第22期5612-5614,5617,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30901025);动物胚胎工程及分子育种湖北省重点实验室课题(2013ZD101);湖北省农业科学院青年基金项目(2013NKYJJ11)

摘　　要：为了检测肉质相关基因TCAP(Titin-cap,Telethonin)启动子与转录因子MyoD(Myogenic differentiation antigen)的体内结合情况,采用染色质免疫共沉淀(Chromatin immunoprecipitation,ChIP)结合PCR技术分析TCAP启动子与转录因子MyoD的结合。结果表明,以MyoD抗体免疫沉淀的DNA片段为模板,PCR扩增获得了TCAP基因启动区121 bp的片段,实现了转录因子MyoD与TCAP启动子DNA序列结合。试验证实TCAP基因是MyoD调控的下游基因,在肌肉发育过程中发挥重要作用。The aim of this research was to identify whether MyoD transcription factor binds with TCAP（Titin-cap, Telethonin） gene promoter. The interaction between MyoD transcription factor and TCAP gene promoter was detected by Chromatin im- munoprecipitation（ChIP） and PCR technologies. The results showed that the specific 121 bp fragment of TCAP gene were de- tected in the DNA fragments immunoprecipitated by antibody specific for MyoD. This indicated that MyoD transcription factor could bind with TCAP gene promoter. The TCAP gene is one of downstream genes regulated by MyoD, thus plays an impor- tant role in the process of muscle development.

关 键 词：染色质免疫共沉淀 TCAP基因 MyoD转录因子 

分 类 号：Q71[生物学—分子生物学]