红芪多糖HPS1-D的化学结构和抗补体活性研究  

Chemical structural features and anti-complementary activity of polysaccharide HPS1-D from Hedysarum polybotrys

作　　者：杨涛[1] 郭龙[1] 李灿[1] 杨英来[1] 封士兰[1] 

机构地区：[1]兰州大学药学院,甘肃兰州730000

出　　处：《中国中药杂志》2014年第1期89-93,共5页China Journal of Chinese Materia Medica

基　　金：国家"十二五"科技支撑计划项目(2011BAI05B02)

摘　　要：目的:红芪多糖HPS1-D的化学结构、初步构象和抗补体活性的研究。方法:红芪经水提醇沉法提取、Sevage法脱蛋白、H2O2脱色素、SephadexG-100色谱柱分离纯化得到均一的红芪多糖HPS1-D。以GC、高效液相凝胶色谱法(HPLCGPC)、凝胶渗透色谱-多角度激光散射仪联用法(GPC-MALLS)、元素分析、苯酚硫酸法、Bradford法、硫酸咔唑法研究其理化性质;采用甲基化、部分酸水解、以及NMR研究其连接方式、主链和支链结构及分支点状况;以GPC-MALLS、对其构象进行初步分析;采用细胞溶血法对其抗补体活性进行研究。结果:HPS1-D主要由葡萄糖和阿拉伯糖组成,主链骨架由1,4和1,4,6-α-D-Glcp,侧链分支位于葡萄糖6位;侧链分支主要由1,5和1,3,5-α-L-Araf组成;相对分子质量为5.2×105,在0.9%NaCl溶液中为无规则线团;抗补体实验表明HPS1-D有一定程度的抗补体活性,并呈一定的剂量效应关系。结论:HPS1-D为1种新的中性红芪杂多糖,具有一定的抗补体活性。HPS1-D, an active polysaccharide,was isolated and purified from Hedysarum polybotrys. HPSI-D was obtained after treated with Savage method and H2 02, and purified with DEAE-cellulose 52 and Sephadex G-100 gel filtration chromatography. Then physicochemical property analysis, GC, methylation, partial acid hydrolysis, and NMR method were used to study chemical structural of HPS1-D. The conformation was primarily analyzed with GPC-MALLS method and Congo red reaction. The anti-complementary activity of HPS1-D was evaluated with the hemolysis assay. HPS1-D was a heteropolysaceharide and consisted of D-glucose, L-arab inose, （7. 2： 1.3）. HPS1-D proved to be a neutral sugar, with 1,4-and 1,4,6-a-D-glucopyranosyl residues in backbone ,and 1,5-and 1, 3, 5-a-L-arabinofuranosyl residues in branches. HPS1-D has a random coil state conformation with monodisperse mass distribution in 0. 9% NaCl solution. And HPS1-D had triple-helix conformation in concentrate of NaOH solution . Anti-complementary activity of HPS1-D was closed to its positive control heparin.

关 键 词：红芪 多糖 结构 构象 抗补体 

分 类 号：R284[医药卫生—中药学]