水提三七达玛烷20(S)-原人参二醇型皂苷诱导A549细胞凋亡机制的研究  

Effects of 20(s)-protopanaxadiol on apoptosis of human pulmonary adenocarcinoma A549 cells and its mechanism

作　　者：陈道梅 李晚谊[2] 肖丹[2] 刘玫[3] 李斌[3] 

机构地区：[1]云南大学化学科学与工程学院,云南昆明650091 [2]云南省农科院药物研究所,云南昆明650205 [3]云南大学药学院云南大学生物制药创新人才培养基地,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2014年第1期132-138,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(30560178/H3002);云南省科技计划(2012AE0020)

摘　　要：三七总甙对多种肿瘤细胞的生长均有抑制作用,但从三七茎叶、花梗、果梗提取到的达玛烷20(S)-原人参二醇型皂苷(20(S)-PPDS)对肺癌A549细胞的作用及机理尚不清楚.本研究采用噻唑蓝(MTT),Hoechst33258染色,免疫组化以及Western blot法研究20(S)-PPDS对肺癌A549细胞的作用.结果显示20(S)-PPDS对A549细胞的具有抑制作用,125,250,500μg·mL-1的20(S)-PPDS与A549细胞作用72 h后,细胞抑制率依次为28.67%,41.97%,73.02%;20(S)-PPDS对A549细胞作用24,72 h的IC50分别为398.94,315.96μg·mL-1;20(S)-PPDS通过活化Caspase-3,上调Bax蛋白表达,下调Pro-Caspase-3、Bcl-2蛋白表达诱导A549细胞凋亡.这些研究结果表明20(S)-PPDS对A549细胞具有抑制细胞增殖和诱导细胞凋亡的作用.Panax notogingseng saponins inhibits the growth of several cancer cell lines, but the inhibition of 20 （ S ） - protopanaxadiol saponins （ 20 （S） - PPDS） , an aqueous extraction group from stem, leaves, pedicel, and fruit stem of Pannax notoginseng, on the proliferation of lung adenocarcinoma cell line A549 and its possible mo- lecular mechanism is not clear. After treating A549 cells with 20 （S） - PPDS, we detected the effects of 20 （S） - PPDS on A549 cells by MTT assay,immunohistochemistry, Hoechst33258 staining, and Westem blot. The results showed that 20（S） -PPDS inhibited the growth of A549 cells. When treated with 125,250 and 500 μg mL-1 of 20 （S） - PPDS for 72 h, the inhibitory rates of A549 cells were 28.67%, 41.97%, 73.02% respectively. The 50% inhibition concentration of 20（S） - PPDS were 398.94 and 315.90 μg mL-1 at 24 and 72 h. 20（S） - PPDS induced cell apoptosis through activating Caspase - 3, up - regulating Bax, down - regulating Pro - Caspase - 3 and Bcl - 2 protein expression. Experimental results of this study suggested that 20 （S） - PPDS exerts anti- cancer activity through the growth inhibition and the apoptosis on A549 cells.

关 键 词：三七20(S)-原人参二醇型皂苷 肺癌 A549细胞株 凋亡 Pro-Caspase-3 BAX Bcl-2 

分 类 号：R285.5[医药卫生—中药学]