甘蔗黑腐病病原菌的鉴定  

Identification of the pathogen causing black rot in sugarcane (Saccharum sinensis)

作　　者：李婕[1] 李永川 杨虹 余磊[3] 黄琼[1] 

机构地区：[1]云南农业大学植物保护学院农业生物多样性应用技术国家工程研究中心,云南昆明650201 [2]云南省植保质检站,云南昆明650034 [3]昆明学院农学院云南省高校都市型现代农业工程研究中心,云南昆明650214

出　　处：《云南大学学报（自然科学版）》2014年第1期139-143,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金(31270067);云南省教育厅科学研究基金(2011Z032)

摘　　要：在云南开远6根发病的甘蔗茎上分离到甘蔗黑腐病病原菌,鉴定了该病害的病原菌,以期为防治和控制该病害提供一定的理论依据.基于致病性测定,ITSI-5.8S-ITS2 rDNA序列的系统发病分析和形态学观察结果,甘蔗黑腐病是由多脂长喙壳菌(Ceratocystis adiposa(Butl.)Moreau)引起的,该病菌导致甘蔗黑腐病在中国属首次报道.Six diseased sugarcane （ Saccharum Province and the pathogen were isolated from roots the disease, and provide a theoretical basis for the sinensis） samples were collected in Kaiyuan City of Yunnan of infected sugarcane plant. In order to clear the pathogen of prevention and control of the disease, we identified the patho- gen of this disease. Based on the pathogenicity, analysis of the rDNA internal transcribed spacer region （ ITSI - 5. 8S- ITS2） and morphology,the results showed that they were the pathogens of sugarcane （Saccharum. sinensis） of black rot disease caused by Ceratocystis adiposa. This was the first reported of Ceratocystis adiposa as the patho- gen of black rot of sugarcane in China.

关 键 词：甘蔗 黑腐病 致病性 ITS 

分 类 号：S432.42[农业科学—植物病理学]