栽培与野生羌活中4种化合物含量及抗炎作用比较  

Comparison of Anti-inflammatory Effects and the Contents of 4 Compounds from Cultivated and Wild Notopterygii Rhizoma Et Radix

作　　者：王冬梅[1] 王珍[1] 黄林芳[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193

出　　处：《中国药房》2014年第3期199-202,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81274013;81130069);863计划课题(No.2010AA023006);国家重大科技专项创制新药课题(No.2011ZX09307-002-01);人事部留学人员择优资助项目(No.2009-2012)

摘　　要：目的:比较栽培与野生羌活中4种化合物含量以及抗炎作用。方法:应用高效液相色谱(HPLC)法测定栽培与野生羌活中羌活醇、异欧前胡素、阿魏酸、佛手柑内酯的含量。复制二甲苯致小鼠耳廓肿胀模型和角叉菜胶致大鼠足跖肿胀模型,观察栽培与野生羌活水提液与醇提液对炎症模型动物肿胀度的影响。结果:栽培羌活所含4种成分含量大多高于野生羌活。野生羌活(班玛、壤塘、康定、西宁)、栽培羌活(岷县、渭源)水提液可显著抑制二甲苯致小鼠耳廓肿胀,其中栽培羌活(渭源)水提液效果最好;野生羌活(班玛、阿坝、壤塘、康定、西宁)醇提液、栽培羌活(岷县、渭源)醇提液可显著抑制角叉菜胶致大鼠足跖肿胀,其中栽培羌活(岷县)醇提液效果最好。结论:栽培羌活在化学成分含量及抗炎效果方面均等同于或强于野生品。OBJECTIVE： To compare the contents and the anti-inflammatory effects of 4 components in wild and cultured Notopterygii Rhizoma Et Radix. METHODS： HPLC method was used to determine the contents of notopterol, isoimperatorin, ferulic acid and bergapten in cultured and wild Notopterygii Rhizoma Et Radix. The carrageenin-induced paw edema rat model and the xylene-induced ear edema mice model were established, and the effects of aqueous extract and ethanol extract of cultured and wild Notopterygii Rhizoma Et Radix on swelling degree of inflammation models were observed. RESULTS. Most of the contents of 4 components in the cultivated Notopterygii Rhizoma Et Radix were higher than in the wild. Wild Notopterygii Rhizoma Et Radix （from B anma）, wild Notopterygii Rhizoma Et Radix （from Rangtang）, wild Notopterygii Rhizoma Et Radix （from Kangding）, wild Notopterygii Rhizoma Et Radix （from Xining）, cultivated Notopterygii Rhizoma Et Radix （from Minxian）, cultivated Notopterygii Rhizoma Et Radix（from Weiyuan） water extract inhibitted the xylene induced mouse auricle swelling, The cultivated Notopterygii Rhizoma Et Radix （from Weiyuan） water extract had the best effect. Wild Notopterygii Rhizoma Et Radix （from Banma）, wild Notopterygii Rhizoma Et Radix（from Aba）, wild Notopterygii Rhizoma Et Radix（from Rangtang）, wild Notopterygii Rhizoma Et Radix （from Kangding）, wild Notopterygii Rhizoma Et Radix （from Xining）, cultivated Notopterygii Rhizoma Et Radix （from Minxian）, cultivated Notopterygii Rhizoma Et Radix（from Weiyuan） ethanol extract inhibitted the xylene induced mouse auricle swelling, The cultivated Notopterygii Rhizoma Et Radix （from Minxian） ethanol extract had the best effect. CONCLUSIONS： Anti-inflammatory activities of cultivated Notopterygii Rhizoma Et Radix are equal to or better than other wild ones.

关 键 词：栽培 野生 羌活 化学成分 抗炎作用 提取物 

分 类 号：R285[医药卫生—中药学] R284[医药卫生—中医学]