海藻玉壶汤加减方对自身免疫性甲状腺炎模型大鼠的保护作用  

Protective Effect of Modified Haizao Yuhu Decoction on Autoimmune Thyroiditis Model Rats

作　　者：陈然峰[1] 田港[2] 张小燕[2] 唐晓霞[2] 宋霄宏[2] 

机构地区：[1]武警浙江省总队杭州医院,杭州310051 [2]浙江中医药大学药学院,杭州310053

出　　处：《中国药房》2014年第3期215-217,共3页China Pharmacy

基　　金：浙江省自然科学基金资助项目(No.Y2110967);浙江省大学生科技创新活动计划暨新苗人才计划项目(No.2012R410058)

摘　　要：目的:研究海藻玉壶汤加减方对自身免疫性甲状腺炎(EAT)模型大鼠的保护作用。方法:多点皮下注射猪甲状腺球蛋白(PTg)进行初次免疫,于第2周进行加强免疫,以后每周加强免疫1次,共加强免疫4次,以复制大鼠EAT模型。70只SD大鼠分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、丙基硫氧嘧啶(23.43 mg/kg)组、雷公藤多苷(6.25 mg/kg)组、夏枯草(1.875g/kg)组与海藻玉壶汤加减方高、低剂量(39.4、19.7 g/kg)组。灌胃给药,每天1次,连续16周。末次给药后大鼠眼眶取血测定血清三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)含量与甲状腺球蛋白抗体(TGAb)、甲状腺过氧化物酶抗体(TPOAb)活性;取甲状腺,称定质量,计算甲状腺脏器指数(甲状腺质量/体质量)。结果:与正常对照组比较,模型组大鼠T3、T4含量增加,TGAb活性增强,甲状腺脏器指数升高,差异具有统计学意义(P<0.01或P<0.05)。与模型组比较,海藻玉壶汤加减方高、低剂量组大鼠TGAb活性减弱,甲状腺系数降低,差异具有统计学意义(P<0.01或P<0.05);海藻玉壶汤加减方高剂量组大鼠T4含量减少,差异具有统计学意义(P<0.05)。结论:海藻玉壶汤加减方能调节EAT模型大鼠的甲状腺激素及抗体的水平。OBJECTIVE： To observe the protective effect of Modified haizao yuhu decoction on autoimmune thyroiditis （EAT） model rat. METHODS： Hypodermic injection of thyroglobulin （PTg） at multi-sites was carried out for primary immunity. Supplementary immunization was conducted in second week and then strengthened once a week. EAT models were established after 4 times of supplementary immunization. 70 SD rats were divided into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, propylthiouracide group （23.43 mg/kg）, tripterygium glycosides group （6.25 mg/kg）, Prunella vulgaris group （1.875 g/kg） and Modified haizao yuhu decoction high-dose and low-dose groups （39.4 g/kg, 19.7 g/kg）. They were given relevant medicines intragastrically once a day for consecutive 16 weeks. After last medication, orbital blood was collected and T3, T4, TGAb and TPOAb in serum of rats were measured. The thyroid gland was sampled and weighted, and then thyroid viscera index（thyroid weight/body weight） were calculated. RESULTS： Compared with normal control group, the contents of T3 and T4, TGAb and thyroid index were increased significantly in model group（P〈0.01）; compared with model group, the con- tents of TGAb and thyroid index were decreased significantly in Modified haizao yuhu decoction high-dose and low-dose groups （P〈0.01 or P〈0.05）, the content of T4 was decreased signifiantly in Modified haizaoyuhu decoction high-dose group（P〈0.05）. CONCLUSIONS： Modified haizao yuhu decoction can adjust the thyroid hormone and antibody in the EAT rats.

关 键 词：海藻玉壶汤加减方 自身免疫性甲状腺炎 三碘甲状腺原氨酸 四碘甲状腺原氨酸 甲状腺球蛋白抗体 甲状腺过氧化物酶抗体 

分 类 号：R285.5[医药卫生—中药学] R593.2[医药卫生—中医学]