根癌农杆菌介导Bt杀虫基因对花椰菜的转化初报  

Preliminary Report of Bt Infected in Cauliflower by Agrobacterium Tumefaciens

作　　者：蔡荣旗[1] 孙德岭[1] 赵前程[1] 李素文[1] 张宝珍[1] 方文惠[1] 

机构地区：[1]天津市蔬菜研究所,天津300384

出　　处：《天津农业科学》2000年第4期9-12,共4页Tianjin Agricultural Sciences

基　　金：天津市自然基金项目 !(98380 2 0 11)

摘　　要：采用 Ti质粒 p KSB带有 Bt杀虫蛋白基因、NPT 选择标记基因及 GUS基因 ,通过根癌农杆菌 L BA40 44菌株的介导 ,将 Bt杀虫基因导入花椰菜品种中。试验以花椰菜下胚轴为外植体 ,将下胚轴切段置于分化培养基 (MS+ 6 - BA1 .0～ 2 .0mg/L + NAA 0～ 0 .5 mg/L)上 ,对影响转化的种种因素进行了试验。得到了转化的愈伤组织及再生植株 ,用组织化学法检测 GUS活性 。Bt(Baillus thuringiensis)NPTⅡ selective mark and GUS gene in Tiplasmid was infected in cauliflower(Brassica oleracea var.botrytis) by Agrobacterium Tumefaciens LBA4044.Explant was hypocotyl of seedlings,factors effecting on introduction was studied.The introduced callus and regenerated plants was obtained.Expression of GUS was detected by histochemical mathod.Blue was found in transformed cell of callus.

关 键 词：花椰菜 根癌农杆菌 BT杀虫基因 抗虫育种 

分 类 号：S635.303.4[农业科学—蔬菜学] S188[农业科学—园艺学]